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Anti-Human PMEL Polyclonal Antibody (PHE28701)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P40967
Overview

Catalog No.

PHE28701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PMEL (Gln65-Thr299).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ME20M, Melanocyte protein PMEL, Silver locus protein homolog, Secreted melanoma-associated ME20 antigen, D12S53E, 95 kDa melanocyte-specific secreted glycoprotein, SILV, P100, Premelanosome protein, PMEL17, ME20S, PMEL, ME20-S, ME20-M, P26, P1, Melanocytes lineage-specific antigen GP100, Melanocyte protein Pmel 17, Melanoma-associated ME20 antigen

Purification

Purified by antigen affinity column.

Accession

P40967

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PMEL antibody (PHE28701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with PMEL antibody (PHE28701) at 1 μg/ml.

    Lane 1: Rat heart
    Lane 2: Mouse heart

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 70 kDa
    Observed MW: 70 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human skin tissue stained for PMEL with PHE28701.
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human PMEL Polyclonal Antibody [PHE28701]
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