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Anti-Human ATG14 Polyclonal Antibody

Catalog #:   PHK40801 Specific References (9) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q6ZNE5
Overview

Catalog No.

PHK40801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATG14 (Glu209-Glu415).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000

Target

ATG14L, ATG14, Barkor, Atg14L, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, KIAA0831

Concentration

1 mg/ml

Purification

Purified by antigen affinity column.

Accession

Q6ZNE5

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ATG14 antibody (PHK40801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ATG14 antibody (PHK40801) at 1 μg/ml.

    Lane 1: Rat brain
    Lane 2: Hela

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 55 kDa
    Observed MW: 57 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat brain stained for ATG14 with PHK40801.
References

MEHP induced mitochondrial damage by promoting ROS production in CIK cells, leading to apoptosis, autophagy, cell cycle arrest., PMID:39586385

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545

PDPK1 regulates autophagosome biogenesis by binding to PIK3C3., PMID:32876514

PKD2/polycystin-2 induces autophagy by forming a complex with BECN1., PMID:32543276

Binding of Avibirnavirus VP3 to the PIK3C3-PDPK1 complex inhibits autophagy by activating the AKT-MTOR pathway., PMID:31885313

Disrupted apolipoprotein L1-miR193a axis dedifferentiates podocytes through autophagy blockade in an APOL1 risk milieu., PMID:31116585

Atg14 protects the intestinal epithelium from TNF-triggered villus atrophy., PMID:30894050

Autophagy Is an Innate Mechanism Associated with Leprosy Polarization., PMID:28056107

ULK1-mediated phosphorylation of ATG14 promotes autophagy and is impaired in Huntington's disease models., PMID:27938392

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human ATG14 Polyclonal Antibody [PHK40801]
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