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Anti-Human CYC1 Polyclonal Antibody

Catalog #:   PHC33901 Specific References (26) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P08574
Overview

Catalog No.

PHC33901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYC1 (Arg55-Gly217).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Complex III subunit 4, Cytochrome b-c1 complex subunit 4, Cytochrome c1, heme protein, mitochondrial, CYC1, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Complex III subunit IV, Cytochrome c-1

Purification

Purified by antigen affinity column.

Accession

P08574

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with CYC1 antibody (PHC33901) at 1μg/ml.

    Lane 1: A549 cell lysate
    Lane 2: A375 cell lysate
    Lane 3: LO2 cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: Mouse kidney lysate
    Lane 6: Mouse liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 35 kDa
    Observed MW: 31 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human kidney stained for CYC1 with PHC33901.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CYC1 antibody (PHC33901) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
References

Sirt1 Promotes a Thermogenic Gene Program in Bone Marrow Adipocytes: From Mice to (Wo)Men., PMID:30873124

Chromogranin A regulates vesicle storage and mitochondrial dynamics to influence insulin secretion., PMID:28220294

Specific electron transport chain abnormalities in amyotrophic lateral sclerosis., PMID:19240958

Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing., PMID:16954299

A relation between the principal axes of inertia and ligand binding., PMID:10655470

Two distinct classes of mitotic cyclin homologues, Cyc1 and Cyc2, are involved in cell cycle regulation in the ciliate Paramecium tetraurelia., PMID:10568031

Antibody immunodiversity: a study on the marked specificity difference between two anti-yeast iso-1 cytochrome c monoclonal antibodies whose epitopes are closely related., PMID:10524770

The Saccharomyces cerevisiae peroxisomal 2,4-dienoyl-CoA reductase is encoded by the oleate-inducible gene SPS19., PMID:9268358

PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I., PMID:9032237

Differential functional activities of rainbow trout and human estrogen receptors expressed in the yeast Saccharomyces cerevisiae., PMID:7588805

Isolation of a mitotic-like cyclin homologue from the protozoan Trypanosoma brucei., PMID:8406045

Evidence for an interaction between the CYP1(HAP1) activator and a cellular factor during heme-dependent transcriptional regulation in the yeast Saccharomyces cerevisiae., PMID:8458333

Protein A antibody-capture ELISA (PACE): an ELISA format to avoid denaturation of surface-adsorbed antigens., PMID:8381447

Mapping antibody binding sites on cytochrome c with synthetic peptides: are results representative of the antigenic structure of proteins?, PMID:7680266

Axonal trajectories and distribution of GABAergic spinal neurons in wildtype and mutant zebrafish lacking floor plate cells., PMID:1479075

A study of hydrogen exchange of monoclonal antibodies: specificity of the antigen-binding induced conformational stabilization., PMID:1327157

The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes., PMID:1511691

A study of core domains, and the core domain-domain interaction of cytochrome c fragment complex., PMID:1376596

Mammalian p53 can function as a transcription factor in yeast., PMID:1579447

On the hypothetical protein F154 of the TTV1 virus from Thermoproteus tenax. Part III: Immunological identification of the protein with anti-peptide antibodies., PMID:1691001

Expression of functional chicken oviduct progesterone receptors in yeast (Saccharomyces cerevisiae)., PMID:2689442

Identification of a yeast protein with properties similar to those of the immunoglobulin heavy-chain enhancer-binding protein NF-muE3., PMID:2511431

Translation in Saccharomyces cerevisiae: initiation factor 4A-dependent cell-free system., PMID:2668952

Biochemical and immunological characterization of the bovine leukemia virus (BLV) envelope glycoprotein (gp51) produced in Saccharomyces cerevisiae., PMID:2551774

Studies on immunoassays of peptide factors. III. Gastrin/iso-1-cytochrome C as immunogen for raising anti-gastrin antisera., PMID:3040037

Differential accumulation of two apo-iso-cytochromes c in processing mutants of yeast., PMID:6286634

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Human CYC1 Polyclonal Antibody [PHC33901]
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