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Anti-Human ATOH1 Polyclonal Antibody

Catalog #:   PHK39301 Specific References (24) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q92858
Overview

Catalog No.

PHK39301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATOH1 (Ser150-Thr217).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Helix-loop-helix protein hATH-1, BHLHA14, Class A basic helix-loop-helix protein 14, ATOH1, ATH1, Protein atonal homolog 1, bHLHa14, hATH1

Purification

Purified by antigen affinity column.

Accession

Q92858

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ATOH1 antibody (PHK39301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ATOH1 antibody (PHK39301) at 1 μg/ml.

    Lane 1: Mouse kidney

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 38 kDa
    Observed MW: 38 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse cochlea stained for ATOH1 with PHK39301.
References

Administration of cyclophosphamide to rats induces pica and potentiates 5-hydroxytryptamine synthesis in the intestine without causing severe intestinal injury., PMID:34507634

Characterization of spatial and temporal development of Type I and Type II hair cells in the mouse utricle using new cell-type-specific markers., PMID:30455179

Multiple zebrafish atoh1 genes specify a diversity of neuronal types in the zebrafish cerebellum., PMID:29548943

The role of Gαq/Gα11 signaling in intestinal epithelial cells., PMID:29387814

Requirement of Gαq/Gα11 Signaling in the Preservation of Mouse Intestinal Epithelial Homeostasis., PMID:28174748

Hair cell regeneration or the expression of related factors that regulate the fate specification of supporting cells in the cochlear ducts of embryonic and posthatch chickens., PMID:26688175

In Vivo Cochlear Hair Cell Generation and Survival by Coactivation of β-Catenin and Atoh1., PMID:26224861

Embryonic maturation of epidermal Merkel cells is controlled by a redundant transcription factor network., PMID:25468937

In vivo generation of immature inner hair cells in neonatal mouse cochleae by ectopic Atoh1 expression., PMID:24586731

[Transdifferention of some supporting cells in the cochlea induced by Ad5 atoh1/EGFP in the young adult guinea pigs]., PMID:22870721

NOTCH Signaling and ATOH1 in Colorectal Cancers., PMID:21980310

Molecular characterization of the mouse superior lateral parabrachial nucleus through expression of the transcription factor Runx1., PMID:21085653

Neurod1 suppresses hair cell differentiation in ear ganglia and regulates hair cell subtype development in the cochlea., PMID:20661473

Expression of Reg IV and Hath1 in neuroendocrine neoplasms., PMID:19924642

Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis., PMID:19786578

Epithelial stem cell-related alterations in Trichinella spiralis-infected small intestine., PMID:19397593

Voltage-activated ion channels and Ca(2+)-induced Ca (2+) release shape Ca (2+) signaling in Merkel cells., PMID:18415122

Cooperation between the Hic1 and Ptch1 tumor suppressors in medulloblastoma., PMID:18347096

The α1 subunit of nicotinic acetylcholine receptors in the inner ear: transcriptional regulation by ATOH1 and co-expression with the γ subunit in hair cells., PMID:17961150

Bone marrow mesenchymal stem cells are progenitors in vitro for inner ear hair cells., PMID:17113786

Mapping of notch activation during cochlear development in mice: implications for determination of prosensory domain and cell fate diversification., PMID:16736472

A novel role for the choroid plexus in BMP-mediated inhibition of differentiation of cerebellar neural progenitors., PMID:16325379

Crossinhibitory activities of Ngn1 and Math1 allow specification of distinct dorsal interneurons., PMID:11502254

Regulated expression of neurogenic basic helix-loop-helix transcription factors during differentiation of the immortalized neuronal progenitor cell line HC2S2 into neurons., PMID:9634594

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human ATOH1 Polyclonal Antibody [PHK39301]
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