Catalog No.
PHG46201
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human MAD2L1 (Met1-Asp205).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
MAD2L1, Mitotic arrest deficient 2-like protein 1, HsMAD2, MAD2-like protein 1, Mitotic spindle assembly checkpoint protein MAD2A, MAD2
Purification
Purified by antigen affinity column.
Accession
Q13257
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Single-cell analysis of neoplastic plasma cells identifies myeloma pathobiology mediators and potential targets., PMID:39855192
Differential anti-viral response to respiratory syncytial virus A in preterm and term infants., PMID:38447274
Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes., PMID:30464155
MAD2 Combined with Mitotic Spindle Apparatus (MSA) and Anticentromere Antibody (ACA) for Diagnosis of Small Cell Lung Cancer (SCLC)., PMID:30346937
The closed form of Mad2 is bound to Mad1 and Cdc20 at unattached kinetochores., PMID:29895228
BRCA1 and MAD2 Are Coexpressed and Are Prognostic Indicators in Tubo-ovarian High-Grade Serous Carcinoma., PMID:29465507
Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling., PMID:26986935
The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation., PMID:26912231
Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint., PMID:26031201
Deregulation of HMGA1 expression induces chromosome instability through regulation of spindle assembly checkpoint genes., PMID:26009897
Chromosome missegregation rate predicts whether aneuploidy will promote or suppress tumors., PMID:24133140
MYCN-mediated overexpression of mitotic spindle regulatory genes and loss of p53-p21 function jointly support the survival of tetraploid neuroblastoma cells., PMID:23186832
EB1 acetylation by P300/CBP-associated factor (PCAF) ensures accurate kinetochore-microtubule interactions in mitosis., PMID:23001180
Tripeptidyl peptidase II in human oral squamous cell carcinoma., PMID:22986808
Expression of the mitotic-arrest deficiency 2 is associated with chemotherapy resistance in ovarian serous adenocarcinoma., PMID:22797604
Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes., PMID:22513372
Timing of anaphase-promoting complex activation in mouse oocytes is predicted by microtubule-kinetochore attachment but not by bivalent alignment or tension., PMID:22513370
Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint., PMID:21772247
Mps1 at kinetochores is essential for female mouse meiosis I., PMID:21558374
Toward a comprehensive characterization of the phosphotyrosine proteome., PMID:21447384
Effects of artesunate on cytokinesis and G₂/M cell cycle progression of tumour cells and budding yeast., PMID:21156967
MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes., PMID:20406800
Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpoint., PMID:19558549
Spindle assembly checkpoint-related failure perturbs early embryonic divisions and reduces reproductive performance of LT/Sv mice., PMID:19279200
Metaphase I arrest in LT/Sv mouse oocytes involves the spindle assembly checkpoint., PMID:18753610
Perturbation of the chromosomal binding of RCC1, Mad2 and survivin causes spindle assembly defects and mitotic catastrophe., PMID:18712773
BRCA1 is required for meiotic spindle assembly and spindle assembly checkpoint activation in mouse oocytes., PMID:18596218
Aberrant MAD2 expression in soft-tissue sarcoma., PMID:18477210
PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores., PMID:17998396
Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle poles., PMID:17504913
Subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 assessed by immunohistochemistry., PMID:17242465
2-methoxyestradiol induces spindle aberrations, chromosome congression failure, and nondisjunction in mouse oocytes., PMID:17229934
A new Mad2-interacting domain of Cdc20 is critical for the function of Mad2-Cdc20 complex in the spindle assembly checkpoint., PMID:16497171
Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes., PMID:16322543
Frequent mutations of human Mad2, but not Bub1, in gastric cancers cause defective mitotic spindle checkpoint., PMID:16112690
Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores., PMID:15964272
Dynamics of centromere and kinetochore proteins; implications for checkpoint signaling and silencing., PMID:15182667
Intra-oocyte localization of MAD2 and its relationship with kinetochores, microtubules, and chromosomes in rat oocytes during meiosis., PMID:15115722
Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest., PMID:14691134
Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores., PMID:14654001
Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension., PMID:12388768
Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint., PMID:11707408
Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores., PMID:11451998
Mitotic checkpoint proteins HsMAD1 and HsMAD2 are associated with nuclear pore complexes in interphase., PMID:11181178
Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events., PMID:9628895
Cell cycle checkpoints: arresting progress in mitosis., PMID:9103187
Identification of a human mitotic checkpoint gene: hsMAD2., PMID:8824189
For research use only. Not for human or drug use.
