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Anti-Human DHX9 Polyclonal Antibody

Catalog #:   PHG15401 Specific References (16) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q08211
Overview

Catalog No.

PHG15401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DHX9 (Pro351-Glu562).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

DHX9, Leukophysin, LKP, NDH II, ATP-dependent RNA helicase A, NDH2, DEAH box protein 9, DExH-box helicase 9, RNA helicase A, Nuclear DNA helicase II, DDX9

Purification

Purified by antigen affinity column.

Accession

Q08211

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with DHX9 antibody (PHG15401) at 1μg/ml.

    Lane 1: NIH3T3 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Jurkat cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 141 kDa
    Observed MW: 141 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse brain stained for DHX9 with PHG15401.
References

R-Loop Immunoprecipitation: A Method to Detect R-Loop Interacting Factors., PMID:35704194

The Long Linker Region of Telomere-Binding Protein TRF2 Is Responsible for Interactions with Lamins., PMID:33804854

RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage., PMID:29742442

Expanded CUG repeats Dysregulate RNA splicing by altering the stoichiometry of the muscleblind 1 complex., PMID:21900255

Monoclonal antibody specific for Dhx9/NDHII/RHA., PMID:20569003

Do we need new autoantibodies in lupus?, PMID:20537203

High prevalence of autoantibodies to RNA helicase A in Mexican patients with systemic lupus erythematosus., PMID:20064217

Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs., PMID:19029303

Functional characterization of NF-kappaB inhibitor-like protein 1 (NFkappaBIL1), a candidate susceptibility gene for rheumatoid arthritis., PMID:17855452

Autoantibodies to RNA helicase A: a new serologic marker of early lupus., PMID:17265494

Characterization of RNA helicase A as component of STAT6-dependent enhanceosome., PMID:16914450

RNA helicase A in the MEF1 transcription factor complex up-regulates the MDR1 gene in multidrug-resistant cancer cells., PMID:14769796

X-linked immunodeficient mice spontaneously produce lupus-related anti-RNA helicase A autoantibodies, but are resistant to pristane-induced lupus., PMID:12917264

RNA helicase A interacts with dsDNA and topoisomerase IIalpha., PMID:12711669

Sequence-specific DNA binding activity of RNA helicase A to the p16INK4a promoter., PMID:11038348

Nucleolar localization of murine nuclear DNA helicase II (RNA helicase A)., PMID:10413677

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human DHX9 Polyclonal Antibody [PHG15401]
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