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Anti-HSPD1 Polyclonal Antibody

Catalog #:   PHC83701 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P10809
Overview

Catalog No.

PHC83701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPD1 (Ala27-Phe573).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

60 kDa heat shock protein,mitochondrial,HSP-60,HuCHA60,Chaperonin 60,HSP60,Mitochondrial matrix protein P1,Hsp60,HSPD1,60 kDa chaperonin,CPN60,P60 lymphocyte protein,Heat shock protein 60

Purification

Purified by antigen affinity column.

Accession

P10809

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPD1 antibody (PHC83701) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 60 kDa
    Observed MW: 60 kDa
References

Integrative analysis of serum proteomics and transcriptomics in hepatitis C., PMID:40082995

The Spleen Modulates the Balance of Natural and Pathological Autoantibodies in a Mouse Model of Autoimmune Arthritis., PMID:39519235

The mechanism underlying B-cell developmental dysfunction in Kawasaki disease based on single-cell transcriptomic sequencing., PMID:39507528

Autoimmunity to stromal-derived autoantigens in rheumatoid ectopic germinal centers exacerbates arthritis and affects clinical response., PMID:38950333

Pathogenic Autoimmunity in Atherosclerosis Evolves from HSP60-Reactive CD4 + T Cells., PMID:38767798

Plasma cells in human pancreatic ductal adenocarcinoma secrete antibodies against self-antigens., PMID:37751306

Reactivity to neural tissue epitopes, aquaporin 4 and heat shock protein 60 is associated with activated immune-inflammatory pathways and the onset of delirium following hip fracture surgery., PMID:36520371

Comparative systeomics to elucidate physiological differences between CHO and SP2/0 cell lines., PMID:35228567

Are IgG antibodies to heat shock proteins HSP27 and HSP60 useful markers in endometrial cancer and cervical cancer?, PMID:33914318

Immunoproteomics approach revealed elevated autoantibody levels against ANXA1 in early stage gallbladder carcinoma., PMID:33261560

Overexpression of HSP10 correlates with HSP60 and Mcl-1 levels and predicts poor prognosis in non-small cell lung cancer patients., PMID:32986659

Intense immunostaining of heat shock protein 70 within renal interstitium associates with long-term renal survival in an ANCA-associated vasculitis cohort., PMID:32876904

Chlorogenic acid alleviates acetaminophen-induced liver injury in mice via regulating Nrf2-mediated HSP60-initiated liver inflammation., PMID:32603696

A Soluble Fiber Diet Increases Bacteroides fragilis Group Abundance and Immunoglobulin A Production in the Gut., PMID:32332136

Heat Shock Protein 60 (HSP60) Serves as a Potential Target for the Sensitization of Chemoresistant Ovarian Cancer Cells., PMID:32124395

Using recursive partitioning approach to select tumor-associated antigens in immunodiagnosis of gastric adenocarcinoma., PMID:30950146

Autoantibody Biomarker Discovery in Primary Open Angle Glaucoma Using Serological Proteome Analysis (SERPA)., PMID:30899261

Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H. pylori-infected patients., PMID:30746790

CIGB-814, an altered peptide ligand derived from human heat-shock protein 60, decreases anti-cyclic citrullinated peptides antibodies in patients with rheumatoid arthritis., PMID:30415439

Concentration-dependent protein loading of extracellular vesicles released by Histoplasma capsulatum after antibody treatment and its modulatory action upon macrophages., PMID:29795301

Comparison of T Helper Cell Patterns in Primary Open-Angle Glaucoma and Normal-Pressure Glaucoma., PMID:29616680

Augmented oxidative stress in infertile women with persistent chlamydial infection., PMID:28336198

Evaluation of the relationship between salivary concentration of anti-heat shock protein immunoglobulin and clinical manifestations of Behçet's disease., PMID:28276957

Ebola virus infection induces autoimmunity against dsDNA and HSP60., PMID:28181533

Autoimmunity to HSP60 during diet induced obesity in mice., PMID:27899808

[Studies on immunogenicity and immunoprotection induced by heat shock protein 60 kDa of Schistosoma japonicum in mice]., PMID:27356404

[Role of heat shock proteins in the cardioprotection of regular moderate alcohol consumption]., PMID:26902796

Tolerogenic responses of CD206+, CD83+, FOXP3+, and CTLA-4 to sericin/polyvinyl alcohol/glycerin scaffolds relevant to IL-33 and HSP60 activity., PMID:26864661

[ANTI-Hsp60 ANTIBODIES IN ARTERIAL HYPERTENTION DIFERENT DEGREE OF SEVERITY]., PMID:26827438

Antibodies against small heat-shock proteins in Alzheimer's disease as a part of natural human immune repertoire or activation of humoral response?, PMID:26566902

[Validity of the Morawietz classification for evaluation of periprosthetic tissue]., PMID:26317183

Up-regulated TLR4 in cardiomyocytes exacerbates heart failure after long-term myocardial infarction., PMID:26290459

Anti-citrullinated protein antibodies promote apoptosis of mature human Saos-2 osteoblasts via cell-surface binding to citrullinated heat shock protein 60., PMID:26275591

Mycobacterial heat shock protein 65 (mbHSP65)-induced atherosclerosis: Preventive oral tolerization and definition of atheroprotective and atherogenic mbHSP65 peptides., PMID:26233917

Methylation of Gata3 protein at Arg-261 regulates transactivation of the Il5 gene in T helper 2 cells., PMID:25861992

[Antibodies to Hsp60 in newborn babies, to whom during the intervention for critical inborn heart failure, autologous cord blood was transfused, in early and late postoperative period]., PMID:25842882

TLR4 mutation and HSP60-induced cell death in adult mouse cardiac myocytes., PMID:25716072

Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease., PMID:25641213

Influence of intimal Chlamydophila pneumoniae persistence on cardiovascular complications after coronary intervention., PMID:25344890

Expression and regulation of toll-like receptors (TLRs) in human intervertebral disc cells., PMID:24997157

Recurrent miscarriages, innate immunity, and autoimmune reaction to chlamydial 60-kDa heat shock protein--is there an association?, PMID:24680363

Evidence for a role of autoantibodies to heat shock protein 60, 70, and 90 in patients with dermatitis herpetiformis., PMID:24643797

Antibodies against Hsp60 and Hsp65 in the sera of women with ovarian cancer., PMID:24618330

Immunotherapy of tuberculosis with Mycobacterium leprae Hsp65 as a DNA vaccine triggers cross-reactive antibodies against mammalian Hsp60 but not pathological autoimmunity., PMID:24607935

Antibodies directed to the gram-negative bacterium Neisseria gonorrhoeae cross-react with the 60 kDa heat shock protein and lead to impaired neurite outgrowth in NTera2/D1 cells., PMID:24577885

Barberry treatment reduces serum anti-heat shock protein 27 and 60 antibody titres and high-sensitivity c-reactive protein in patients with metabolic syndrome: a double-blind, randomized placebo-controlled trial., PMID:24536039

Immune markers in oral discomfort patients before and after elimination of oral galvanism., PMID:24522020

The 60- and 70-kDa heat-shock proteins and their correlation with cardiovascular risk factors in postmenopausal women with metabolic syndrome., PMID:24327239

Elevated blood Hsp60, its structural similarities and cross-reactivity with thyroid molecules, and its presence on the plasma membrane of oncocytes point to the chaperonin as an immunopathogenic factor in Hashimoto's thyroiditis., PMID:24057177

IgG subclass antibodies to human and bacterial HSP60 are not associated with disease activity and progression over time in axial spondyloarthritis., PMID:23705835

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-HSPD1 Polyclonal Antibody [PHC83701]
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