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Anti-HSPD1 Polyclonal Antibody (PHC83701)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P10809
Overview

Catalog No.

PHC83701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPD1 (Ala27-Phe573).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

60 kDa heat shock protein,mitochondrial,HSP-60,HuCHA60,Chaperonin 60,HSP60,Mitochondrial matrix protein P1,Hsp60,HSPD1,60 kDa chaperonin,CPN60,P60 lymphocyte protein,Heat shock protein 60

Purification

Purified by antigen affinity column.

Accession

P10809

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with HSPD1 antibody (PHC83701) at 0.28μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: Hela cell lysate
    Lane 3: Mouse heart lysate
    Lane 4: Rat heart lysate
    Lane 5: Rat berebrum lysate
    Lane 6: Rat cerebellum lysate
    Lane 7: Mouse kidney lysate
    Lane 8: Rat kidney lysate
    Lane 9: Rat liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 61 kDa
    Observed MW: 61 kDa
  • Immunocytochemistry/ Immunofluorescence
    HSPD1 in SKOV3 Cell Line.
    The SKOV3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with HSPD1 (PHC83701) at 5.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-HSPD1 Polyclonal Antibody [PHC83701]
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