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Anti-Human ATP5F1B Polyclonal Antibody

Catalog #:   PHC20301 Specific References (21) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P06576
Overview

Catalog No.

PHC20301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP5F1B (Thr60-Ser528).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ATPSB, ATP synthase F1 subunit beta, ATP5F1B, ATP5B, ATPMB, ATP synthase subunit beta, mitochondrial

Purification

Purified by antigen affinity column.

Accession

P06576

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ATP5F1B antibody (PHC20301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 53 kDa
    Observed MW: 53 kDa
References

Synthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein histone H3.1., PMID:36313990

Acquired Resistance to HER2-Targeted Therapies Creates Vulnerability to ATP Synthase Inhibition., PMID:31690671

Mitotane induces mitochondrial membrane depolarization and apoptosis in thyroid cancer cells., PMID:31115496

The role of formation of pyrrole-ATP synthase subunit beta adduct in pyrrolizidine alkaloid-induced hepatotoxicity., PMID:30244272

ATP5A1 and ATP5B are highly expressed in glioblastoma tumor cells and endothelial cells of microvascular proliferation., PMID:26526033

Cross-reactivity of Antibodies Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae With Heat Shock Protein 60 and ATP-Binding Protein Correlates to Reduced Mitochondrial Activity in HIBCPP Choroid Plexus Papilloma Cells., PMID:26080747

Involvement of ATP synthase β subunit in chikungunya virus entry into insect cells., PMID:25168043

Acute epididymitis induces alterations in sperm protein composition., PMID:24726218

Identification of ATP synthase as a lipid peroxide protein adduct in pancreatic islets from humans with and without type 2 diabetes mellitus., PMID:23463654

Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross-reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins., PMID:23335350

A monoclonal antibody against F1-F0 ATP synthase beta subunit., PMID:23098302

Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells., PMID:22753871

miR-127-5p targets the 3'UTR of human β-F1-ATPase mRNA and inhibits its translation., PMID:22433606

Auto-antibodies to β-F1-ATPase and vimentin in malignant mesothelioma., PMID:22022619

A novel mechanism of γ/δ T-lymphocyte and endothelial activation by shear stress: the role of ecto-ATP synthase β chain., PMID:21193741

Autoantibodies to the adenosine triphosphate synthase play a pathogenetic role in Alzheimer's disease., PMID:20594618

[Preparation of monoclonal antibody against lung cancer and identification of its targeting antigen]., PMID:20337043

Human brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH., PMID:20067530

Identification of ATP synthase beta subunit (ATPB) on the cell surface as a non-small cell lung cancer (NSCLC) associated antigen., PMID:19144153

Involvement of cell surface ATP synthase in flow-induced ATP release by vascular endothelial cells., PMID:17545472

Intracellular signaling for vasoconstrictor coupling factor 6: novel function of beta-subunit of ATP synthase as receptor., PMID:16230521

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human ATP5F1B Polyclonal Antibody [PHC20301]
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