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Anti-SARS-CoV-2 ORF3a Polyclonal Antibody

Catalog #:   PVV22301 Specific References (40) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P0DTC3
Overview

Catalog No.

PVV22301

Species reactivity

Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SARS-CoV-2 ORF3a (Leu115-Leu275).

Tested applications

ELISA: 1:4000-1:8000, WB: 1:1000-1:4000

Target

ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a

Purification

Purified by antigen affinity column.

Accession

P0DTC3

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ORF3a antibody (PVV22301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
References

Novel Vaccines Targeting the Highly Conserved SARS-CoV-2 ORF3a Ectodomain Elicit Immunogenicity in Mouse Models., PMID:40266087

A live attenuated SARS-CoV-2 vaccine constructed by dual inactivation of NSP16 and ORF3a., PMID:40132472

Proteome-Wide Analysis of Antibody Responses in Asymptomatic Omicron BA.2-Infected Individuals at the Amino Acid Resolution., PMID:39661118

MDA5 Is a Major Determinant of Developing Symptoms in Critically Ill COVID-19 Patients., PMID:39460899

Implementation of an Immunoassay Based on the MVA-T7pol-Expression System for Rapid Identification of Immunogenic SARS-CoV-2 Antigens: A Proof-of-Concept Study., PMID:39456680

Prevalence and functional profile of SARS-CoV-2 T cells in asymptomatic Kenyan adults., PMID:37219944

Immunization with Recombinant Accessory Protein-Deficient SARS-CoV-2 Protects against Lethal Challenge and Viral Transmission., PMID:37191507

Preclinical safety assessment of the suction-assisted intradermal injection of the SARS-CoV-2 DNA vaccine candidate pGO-1002 in white rabbit., PMID:36683063

Safety and immunogenicity of the bi-cistronic GLS-5310 COVID-19 DNA vaccine delivered with the GeneDerm suction device., PMID:36592685

A bias of Asparagine to Lysine mutations in SARS-CoV-2 outside the receptor binding domain affects protein flexibility., PMID:36569921

Using Alphafold2 to Predict the Structure of the Gp5/M Dimer of Porcine Respiratory and Reproductive Syndrome Virus., PMID:36361998

Structure of SARS-CoV-2 M protein in lipid nanodiscs., PMID:36264056

Age- and Severity-Associated Humoral Immunity Response in COVID-19 Patients: A Cohort Study from Wuhan, China., PMID:36233840

Preparation and pre-clinical evaluation of flagellin-adjuvanted NOM vaccine candidate formulated with Spike protein against SARS-CoV-2 in mouse model., PMID:36030048

Exposure to avian coronavirus vaccines is associated with increased levels of SARS-CoV-2-cross-reactive antibodies., PMID:35869837

Antibodies targeting conserved non-canonical antigens and endemic coronaviruses associate with favorable outcomes in severe COVID-19., PMID:35738278

SARS-CoV-2 accessory proteins reveal distinct serological signatures in children., PMID:35618731

SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies., PMID:35587364

Immunization with recombinant accessory protein-deficient SARS-CoV-2 protects against lethal challenge and viral transmission., PMID:35313573

Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins., PMID:35216036

Clinical, Virological, Immunological, and Genomic Characterization of Asymptomatic and Symptomatic Cases With SARS-CoV-2 Infection in India., PMID:34993157

Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth., PMID:34847745

Monoclonal Human Antibodies That Recognise the Exposed N and C Terminal Regions of the Often-Overlooked SARS-CoV-2 ORF3a Transmembrane Protein., PMID:34835009

Mapping SARS-CoV-2 Antibody Epitopes in COVID-19 Patients with a Multi-Coronavirus Protein Microarray., PMID:34704808

Potential Molecular Mechanisms of Rare Anti-Tumor Immune Response by SARS-CoV-2 in Isolated Cases of Lymphomas., PMID:34696358

SARS-CoV-2 immune repertoire in MIS-C and pediatric COVID-19., PMID:34611361

Multiepitope Proteins for the Differential Detection of IgG Antibodies against RBD of the Spike Protein and Non-RBD Regions of SARS-CoV-2., PMID:34579223

Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics., PMID:34468954

Dynamic landscape mapping of humoral immunity to SARS-CoV-2 identifies non-structural protein antibodies associated with the survival of critical COVID-19 patients., PMID:34404759

An in-depth in silico and immunoinformatics approach for designing a potential multi-epitope construct for the effective development of vaccine to combat against SARS-CoV-2 encompassing variants of concern and interest., PMID:34352457

Genomic monitoring unveil the early detection of the SARS-CoV-2 B.1.351 (beta) variant (20H/501Y.V2) in Brazil., PMID:34241897

Exploratory analysis to identify the best antigen and the best immune biomarkers to study SARS-CoV-2 infection., PMID:34174875

Cryo-EM structure of SARS-CoV-2 ORF3a in lipid nanodiscs., PMID:34158638

The landscape of antibody binding in SARS-CoV-2 infection., PMID:34143766

SARS-CoV-2 Proteins: Are They Useful as Targets for COVID-19 Drugs and Vaccines?, PMID:33622224

Functional alterations caused by mutations reflect evolutionary trends of SARS-CoV-2., PMID:33580783

Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients., PMID:33516277

The landscape of antibody binding in SARS-CoV-2 infection., PMID:33052349

Non-synonymous mutations of SARS-CoV-2 leads epitope loss and segregates its variants., PMID:33049387

Broad and strong memory CD4 + and CD8 + T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients., PMID:32577665

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-SARS-CoV-2 ORF3a Polyclonal Antibody [PVV22301]
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