Catalog No.
YHK72501
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Thr8-Val302
Predicted molecular weight
35.52 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9NXW9
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
ALKBH4, Lysine-specific demethylase ALKBH4, DNA N6-methyl adenine demethylase ALKBH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, ABH4, Alkylated DNA repair protein alkB homolog 4
ALKBH4 functions as a hypoxia-responsive tumor suppressor and inhibits metastasis and tumorigenesis., PMID:39400679
ALKBH4 impedes 5-FU Sensitivity through suppressing GSDME induced pyroptosis in gastric cancer., PMID:38902235
ALKBH4 is a novel enzyme that promotes translation through modified uridine regulation., PMID:37507018
ABHD4 regulates adipocyte differentiation in vitro but does not affect adipose tissue lipid metabolism in mice., PMID:37352974
ALKBH4 Stabilization Is Required for Arsenic-Induced 6mA DNA Methylation Inhibition, Keratinocyte Malignant Transformation, and Tumorigenicity., PMID:37207134
Sperm-borne microRNA-34c regulates maternal mRNA degradation and preimplantation embryonic development in mice., PMID:37101140
Selected ALKBH dioxygenases are overexpressed in salivary gland tumours., PMID:36459535
N6-methyladenine: A Rare and Dynamic DNA Mark., PMID:36350511
Quantitative proteomics revealed new functions of ALKBH4., PMID:34951099
Inhibition of AlkB Nucleic Acid Demethylases: Promising New Epigenetic Targets., PMID:34792334
ALKBH4 promotes tumourigenesis with a poor prognosis in non-small-cell lung cancer., PMID:33883577
An Adversarial DNA N6-Methyladenine-Sensor Network Preserves Polycomb Silencing., PMID:30982744
The AlkB Family of Fe (II)/Alpha-Ketoglutarate-Dependent Dioxygenases Modulates Embryogenesis through Epigenetic Regulation., PMID:29076432
Alkbh4 and Atrn Act Maternally to Regulate Zebrafish Epiboly., PMID:28924386
QM and QM/MM Methods Compared: Case Studies on Reaction Mechanisms of Metalloenzymes., PMID:26415845
The AlkB Family of Fe(II)/α-Ketoglutarate-dependent Dioxygenases: Repairing Nucleic Acid Alkylation Damage and Beyond., PMID:26152727
Introduction: Metals in Biology: α-Ketoglutarate/Iron-Dependent Dioxygenases., PMID:26152720
Theory uncovers an unusual mechanism of DNA repair of a lesioned adenine by AlkB enzymes., PMID:25203306
ALKBH4 depletion in mice leads to spermatogenic defects., PMID:25153837
ALKBH4-dependent demethylation of actin regulates actomyosin dynamics., PMID:23673617
Human ALKBH4 interacts with proteins associated with transcription., PMID:23145062
Spectroscopic and magnetic studies of wild-type and mutant forms of the Fe(II)- and 2-oxoglutarate-dependent decarboxylase ALKBH4., PMID:21166655
Anandamide biosynthesis catalyzed by the phosphodiesterase GDE1 and detection of glycerophospho-N-acyl ethanolamine precursors in mouse brain., PMID:18227059
Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta-hydrolase 4 in this pathway., PMID:16818490
For research use only. Not for human or drug use.
