Catalog No.
YVV22301
Expression system
E. coli
Species
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Protein length
Leu115-Leu275
Predicted molecular weight
20.79 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P0DTC3
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a
SARS-CoV-2 accessory proteins ORF3a and ORF6 alter the miRNome of human lung epithelial cells., PMID:40402297
SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity., PMID:40355429
Acute COVID-19 and LongCOVID syndrome - molecular implications for therapeutic strategies - review., PMID:40313948
The Role of the Tyrosine-Based Sorting Signals of the ORF3a Protein of SARS-CoV-2 in Intracellular Trafficking and Pathogenesis., PMID:40284965
Novel Vaccines Targeting the Highly Conserved SARS-CoV-2 ORF3a Ectodomain Elicit Immunogenicity in Mouse Models., PMID:40266087
Deep Learning-Based Comparative Prediction and Functional Analysis of Intrinsically Disordered Regions in SARS-CoV-2., PMID:40244295
SIRT2 suppresses aging-associated cGAS activation and protects aged mice from severe COVID-19., PMID:40220296
Detection of SARS-CoV-2 in bone marrow megakaryocytes and elevated emperipolesis in COVID-19 patients with thrombocytopenia., PMID:40199447
A live attenuated SARS-CoV-2 vaccine constructed by dual inactivation of NSP16 and ORF3a., PMID:40132472
Subcellular localization of SARS-CoV-2 E and 3a proteins along the secretory pathway., PMID:40025386
SARS-CoV-2-ORF-3a Mediates Apoptosis Through Mitochondrial Dysfunction Modulated by the K+ Ion Channel., PMID:40004042
Calcium release from damaged lysosomes triggers stress granule formation for cell survival., PMID:39962896
Examining the feasibility of replacing ORF3a with fluorescent genes to construct SARS-CoV-2 reporter viruses., PMID:39937571
SARS-CoV-2 ORF3a accessory protein is a water-permeable channel that induces lysosome swelling., PMID:39905220
Induction of the Inflammasome by the SARS-CoV-2 Accessory Protein ORF9b, Abrogated by Small-Molecule ORF9b Homodimerization Inhibitors., PMID:39902616
A comprehensive review of current insights into the virulence factors of SARS-CoV-2., PMID:39878471
Identification of patient demographic, clinical, and SARS-CoV-2 genomic factors associated with severe COVID-19 using supervised machine learning: a retrospective multicenter study., PMID:39875869
HIV-1 Vpu and SARS-CoV-2 ORF3a proteins disrupt STING-mediated activation of antiviral NF-κB signaling., PMID:39836751
Productive infection of the retinal pigment epithelium by SARS-CoV-2: Initial effects and consideration of long-term consequences., PMID:39712068
Features of Highly Homologous T-Cell Receptor Repertoire in the Immune Response to Mutations in Immunogenic Epitopes., PMID:39684303
Proteome-Wide Analysis of Antibody Responses in Asymptomatic Omicron BA.2-Infected Individuals at the Amino Acid Resolution., PMID:39661118
Manipulation of Host Cholesterol by SARS-CoV-2., PMID:39605369
Phylogeogenomic analysis of the earliest reported sequences of SARS-CoV-2 from 161 countries., PMID:39563179
Complete genome sequencing of SARS-CoV-2 strains that were circulating in Uzbekistan over the course of four pandemic waves., PMID:39561193
Calcium signaling from damaged lysosomes induces cytoprotective stress granules., PMID:39533058
MDA5 Is a Major Determinant of Developing Symptoms in Critically Ill COVID-19 Patients., PMID:39460899
Implementation of an Immunoassay Based on the MVA-T7pol-Expression System for Rapid Identification of Immunogenic SARS-CoV-2 Antigens: A Proof-of-Concept Study., PMID:39456680
FPR1 signaling aberrantly regulates S100A8/A9 production by CD14+FCN1hi macrophages and aggravates pulmonary pathology in severe COVID-19., PMID:39402337
SARS-CoV-2 ORF3a Protein Impairs Syncytiotrophoblast Maturation, Alters ZO-1 Localization, and Shifts Autophagic Pathways in Trophoblast Cells and 3D Organoids., PMID:39386577
SARS-CoV-2 ORF3a induces COVID-19-associated kidney injury through HMGB1-mediated cytokine production., PMID:39345136
Aggravating mechanisms from COVID-19., PMID:39334442
SARS-CoV-2 infection and transmission via the skin to oro-nasal route with the production of bioaerosols in the ferret model., PMID:39292223
SARS-CoV-2 NSP16 promotes IL-6 production by regulating the stabilization of HIF-1α., PMID:39251053
ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay., PMID:39232060
Comparative Atlas of SARS-CoV-2 Substitution Mutations: A Focus on Iranian Strains Amidst Global Trends., PMID:39205305
Interaction between SARS-CoV PBM and Cellular PDZ Domains Leading to Virus Virulence., PMID:39205188
Phylogenetic signatures reveal multilevel selection and fitness costs in SARS-CoV-2., PMID:39132669
Potential Transcriptional Enhancers in Coronaviruses: From Infectious Bronchitis Virus to SARS-CoV-2., PMID:39125583
Oxysterole-binding protein targeted by SARS-CoV-2 viral proteins regulates coronavirus replication., PMID:39119292
T-Cell Epitope Mapping of SARS-CoV-2 Reveals Coordinated IFN-γ Production and Clonal Expansion of T Cells Facilitates Recovery from COVID-19., PMID:39066169
Metabolic and mitochondria alterations induced by SARS-CoV-2 accessory proteins ORF3a, ORF9b, ORF9c and ORF10., PMID:38949191
Outcomes of the EMDataResource cryo-EM Ligand Modeling Challenge., PMID:38918604
SARS-CoV-2 Protein Nsp9 Is Involved in Viral Evasion through Interactions with Innate Immune Pathways., PMID:38911767
T cell hybrid immunity against SARS-CoV-2 in children: a longitudinal study., PMID:38896919
Involvement of SARS-CoV-2 accessory proteins in immunopathogenesis., PMID:38837257
SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity., PMID:38798602
T-cell immunity against Severe Acute Respiratory Syndrome Coronavirus 2 proteins in patients with type 1 diabetes., PMID:38751148
Myelin basic protein antagonizes the SARS-CoV-2 protein ORF3a-induced autophagy inhibition., PMID:38703943
ORF3a of SARS-CoV-2 modulates PI3K/AKT signaling in human lung epithelial cells via hsa-miR-155-5p., PMID:38653431
A mechanism that transduces lysosomal damage signals to stress granule formation for cell survival., PMID:38617306
For research use only. Not for human or drug use.
