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Anti-LDHA Polyclonal Antibody

Catalog #:   PHB85001 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P00338
Overview

Catalog No.

PHB85001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LDHA (Met1-Phe332).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Renal carcinoma antigen NY-REN-59,LDH-A,LDH muscle subunit,LDH-M,Cell proliferation-inducing gene 19 protein,L-lactate dehydrogenase A chain,LDHA

Purification

Purified by antigen affinity column.

Accession

P00338

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with LDHA antibody (PHB85001) at 0.34μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: U937 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 36 kDa
  • Immunocytochemistry/ Immunofluorescence
    LDHA in HaCat Cell Line.
    The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with LDHA (PHB85001) at 6.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with LDHA antibody (PHB85001) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 39 kDa
    Observed MW: 39 kDa
References

Targeted activation of junctional adhesion molecule-like protein+ CD8+ T cells enhances immunotherapy in hepatocellular carcinoma., PMID:40353078

Glycogenosis type XI, a rare association between muscle and skin manifestations - the contribution of proteomics for the understanding of the underlying myopathology., PMID:40033989

Tissue factor targeted near-infrared photoimmunotherapy: a versatile therapeutic approach for malignancies., PMID:39751657

Targeting SRSF10 might inhibit M2 macrophage polarization and potentiate anti-PD-1 therapy in hepatocellular carcinoma., PMID:39223929

Exploration of Immune-Modulatory Effects of Amivantamab in Combination with Pembrolizumab in Lung and Head and Neck Squamous Cell Carcinoma., PMID:38916448

Silencing BMAL1 promotes M1/M2 polarization through the LDHA/lactate axis to promote GBM sensitivity to bevacizumab., PMID:38733825

CircHIF1A induces cetuximab resistance in colorectal cancer by promoting HIF1α-mediated glycometabolism alteration., PMID:38715141

Chicken muscle antibody array reveals the regulations of LDHA on myoblast differentiation through energy metabolism., PMID:37890747

Transcriptomics and cell painting analysis reveals molecular and morphological features associated with fed-batch production performance in CHO recombinant clones., PMID:37555462

Metal-Phenolic Nanomedicines Regulate T-Cell Antitumor Function for Sono-Metabolic Cancer Therapy., PMID:37486249

Distinct metabolic requirements regulate B cell activation and germinal center responses., PMID:37365386

Aspirin induces immunogenic cell death and enhances cancer immunotherapy in colorectal cancer., PMID:37290325

Anti-GD2 immunoliposomes loaded with oxamate for neuroblastoma., PMID:36788290

Lipid nanoparticle-mediated CRISPR/Cas9 gene editing and metabolic engineering for anticancer immunotherapy., PMID:36382304

Obesity accelerates immune evasion of non-small cell lung carcinoma via TFEB-dependent upregulation of Siglec-15 and glycolytic reprogramming., PMID:36150633

Maternal autoantibody profiles as biomarkers for ASD and ASD with co-occurring intellectual disability., PMID:35618885

Hypoxic in vitro culture reduces histone lactylation and impairs pre-implantation embryonic development in mice., PMID:34930415

Targeting Cancer Metabolism Breaks Radioresistance by Impairing the Stress Response., PMID:34359663

Beyond the Lactate Paradox: How Lactate and Acidity Impact T Cell Therapies against Cancer., PMID:34203136

Single-Cell RNA Sequencing Analysis of the Immunometabolic Rewiring and Immunopathogenesis of Coronavirus Disease 2019., PMID:33936072

Risk assessment analysis for maternal autoantibody-related autism (MAR-ASD): a subtype of autism., PMID:33483694

Patients with low nicotinamide N-methyltransferase expression benefit significantly from bevacizumab treatment in ovarian cancer., PMID:33446144

Metabolic pathways underlying GATA6 regulating Trastuzumab resistance in Gastric Cancer cells based on untargeted metabolomics., PMID:33173435

Serum proteomics analysis of candidate predictive biomarker panel for the diagnosis of trastuzumab-based therapy resistant breast cancer., PMID:32887021

Lysine-222 succinylation reduces lysosomal degradation of lactate dehydrogenase a and is increased in gastric cancer., PMID:32859246

Therapeutic efficacy of liposomal Grb2 antisense oligodeoxynucleotide (L-Grb2) in preclinical models of ovarian and uterine cancer., PMID:32754300

Glycolytic competence in gastric adenocarcinomas negatively impacts survival outcomes of patients treated with salvage paclitaxel-ramucirumab., PMID:32372141

Peptides of neuron specific enolase as potential ASD biomarkers: From discovery to epitope mapping., PMID:31812776

Nanoenabled Modulation of Acidic Tumor Microenvironment Reverses Anergy of Infiltrating T Cells and Potentiates Anti-PD-1 Therapy., PMID:30943039

Nuclear lactate dehydrogenase A senses ROS to produce α-hydroxybutyrate for HPV-induced cervical tumor growth., PMID:30356100

Pyruvate dehydrogenase complex and lactate dehydrogenase are targets for therapy of acute liver failure., PMID:29580866

Single-cell transcriptome sequencing reveals that cell division cycle 5-like protein is essential for porcine oocyte maturation., PMID:29222335

Mucosal Antibodies to the C Terminus of Toxin A Prevent Colonization of Clostridium difficile., PMID:28167669

Reduction of ammonia and lactate through the coupling of glutamine synthetase selection and downregulation of lactate dehydrogenase-A in CHO cells., PMID:27704181

Involvement of COX-2 in nickel elution from a wire implanted subcutaneously in mice., PMID:27452194

Dynamic Contrast Enhanced MRI Assessing the Antiangiogenic Effect of Silencing HIF-1α with Targeted Multifunctional ECO/siRNA Nanoparticles., PMID:27264671

Overcoming cetuximab resistance in Ewing's sarcoma by inhibiting lactate dehydrogenase-A., PMID:27222229

Sunitinib activates Axl signaling in renal cell cancer., PMID:26815723

Antibodies against Clonorchis sinensis LDH could cross-react with LDHB localizing on the plasma membrane of human hepatocarcinoma cell SMMC-7721 and induce apoptosis., PMID:26769711

Association of cancer metabolism-related proteins with oral carcinogenesis - indications for chemoprevention and metabolic sensitizing of oral squamous cell carcinoma?, PMID:25048361

Cannabinoid control of brain bioenergetics: Exploring the subcellular localization of the CB1 receptor., PMID:24944910

Increased 8-hydroxy-2'-deoxyguanosine in plasma and decreased mRNA expression of human 8-oxoguanine DNA glycosylase 1, anti-oxidant enzymes, mitochondrial biogenesis-related proteins and glycolytic enzymes in leucocytes in patients with systemic lupus erythematosus., PMID:24345202

Cetuximab reverses the Warburg effect by inhibiting HIF-1-regulated LDH-A., PMID:23920275

Combinatorial engineering of ldh-a and bcl-2 for reducing lactate production and improving cell growth in dihydrofolate reductase-deficient Chinese hamster ovary cells., PMID:21792592

Immunoproteomics of HER2-positive and HER2-negative breast cancer patients with positive lymph nodes., PMID:21568730

Overcoming trastuzumab resistance in breast cancer by targeting dysregulated glucose metabolism., PMID:21498634

Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases., PMID:21392546

Modified house-keeping gene expression in a rat tail compression loading-induced disc degeneration model., PMID:21387398

Molecular cloning, characterization, and immunolocalization of two lactate dehydrogenase homologous genes from Taenia solium., PMID:21331787

Metabolic compartmentalization in the human cortex and hippocampus: evidence for a cell- and region-specific localization of lactate dehydrogenase 5 and pyruvate dehydrogenase., PMID:17521432

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-LDHA Polyclonal Antibody [PHB85001]
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