InVivoMAb Anti-Human CCL8/MCP-2 (Iv0080)

Catalog #: VHF70301 Specific References (0) DATASHEET

Bioactivity for InVivoMAb Anti-Human CCL8/MCP-2 (Iv0080) ?>

Host species: Human

Isotype: IgG1, kappa

Applications: ELISA, Neutralization

Accession: P80075

Overview

Catalog No.

VHF70301

Species reactivity

Human

Host species

Human

Isotype

IgG1, kappa

Clonality

Monoclonal

Applications

ELISA, Neutralization

Target

Monocyte chemoattractant protein 2, Small-inducible cytokine A8, HC14, C-C motif chemokine 8, SCYA10, MCP2, SCYA8, CCL8, Monocyte chemotactic protein 2, MCP-2

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P80075

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Iv0080

Note

For research use only. Not suitable for clinical or therapeutic use.

Data Image

Western Blot

Fig.4 IL-1β induces the expression of DDX21 in CRC via JAK2-STAT3 signaling pathway. (A) p-JAK2, p-STAT3, p-ERK, β-catenin expression in CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells by Western blot analysis. (B) The size and number of spheres of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (C-D) The number of colony formation (C), migration and invasion ability (D) of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (E) Expression of p-JAK2, pSTAT3, p-ERK, β-catenin, stemness markers, and EMA-related markers in CRC cells (HCT116 and HT29) si-NC, si-DDX21, IL-1β-supplemented si-NC, IL-1β-supplemented si-DDX21 CRC cells by Western blot analysis
Data sourced from citation (PMID: 41160952)
Bioactivity

Fig.4 IL-1β induces the expression of DDX21 in CRC via JAK2-STAT3 signaling pathway. (A) p-JAK2, p-STAT3, p-ERK, β-catenin expression in CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells by Western blot analysis. (B) The size and number of spheres of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (C-D) The number of colony formation (C), migration and invasion ability (D) of CRC cells (HCT116 and HT29) alone, IL-1β-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL-1β depleted TAMs-co-cultured CRC cells; (E) Expression of p-JAK2, pSTAT3, p-ERK, β-catenin, stemness markers, and EMA-related markers in CRC cells (HCT116 and HT29) si-NC, si-DDX21, IL-1β-supplemented si-NC, IL-1β-supplemented si-DDX21 CRC cells by Western blot analysis
Data sourced from citation (PMID: 41160952)

References

Datasheet

Document Download

InVivoMAb Anti-Human CCL8/MCP-2 (Iv0080).pdf

$ 268

Product specifications

1 mg 268 5 mg 880 25 mg 2800

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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