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Anti-CD273/PD-L2/PDCD1LG2 Polyclonal Antibody (PHJ48501)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:Q9BQ51
Overview

Catalog No.

PHJ48501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD273/PD-L2/PDCD1LG2 (Leu20-Thr220).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PDCD1 ligand 2,B7-DC,PD-L2,PDCD1LG2,PDCD1L2,PDL2,Butyrophilin B7-DC,Programmed death ligand 2,Programmed cell death 1 ligand 2,CD273,B7DC,PD-1 ligand 2

Purification

Purified by antigen affinity column.

Accession

Q9BQ51

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with CD273 / PD-L2 / PDCD1LG2 antibody (PHJ48501) at 1μg/ml.

    Lane 1: Raji cell lysate
    Lane 2: HepG2 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 32 kDa
  • Immunocytochemistry/ Immunofluorescence
    CD273 / PD-L2 / PDCD1LG2 in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CD273 / PD-L2 / PDCD1LG2 (PHJ48501) at 20 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-CD273/PD-L2/PDCD1LG2 Polyclonal Antibody [PHJ48501]
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