Catalog No.
EBF78301
Description
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
Biological activity
At 25 ° C, reaction buffer 50 mM Tris-HCl (PH 8.0), the amount of enzyme required to digest 50 ug fusion protein containing the digestion site within 16 hours is defined as 1 unit of enzyme activity, which is 1U.
Expression system
Yeast
Species
Bovine
Protein length
Enterokinase is cloned from bovine and expressed in Yeast.
Predicted molecular weight
22.11 kDa
Nature
Recombinant
Target
Enterokinase
Concentration
> 30 KU / mg, 1mg of recombinant bovine enterokinase can cut at least 1.5g fusion protein.
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Applications
Fusion Protein Cleavage,Protein Purification,Protein Digestion
Form
Liquid
Storage buffer
50 mM Tris-HCl, 50%Glycerol, pH 8.0
Experimental Procedure
Digestion reaction condition
Digestion at 25 ℃ for 16 hours
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
For research use only. Not for human or drug use.
