Affiliations
- Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Sciences, Shandong Normal University, Jinan 250014, PR China; College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, PR China.
- Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Sciences, Shandong Normal University, Jinan 250014, PR China.
- Shandong Province Institute for the Control of Agrochemicals, Jinan 250131, PR China.
- College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, PR China. zhixiangxu@sina.com.
- Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Sciences, Shandong Normal University, Jinan 250014, PR China. zhanghongyan@sdnu.edu.cn.
PMID: 36115225 DOI: 10.1016/j.foodchem.2022.134084
Abstract
Background: Competitive enzyme-linked immunosorbent assays (ELISA) typically use homologous labeled competitors. This study explored improving detectability by using competitors with weak competitiveness for common estrogen disrupting chemicals (EDCs).
Methods: Three estrogen-enzyme conjugates with different responses were tested as competitive molecules in dual-estrogen receptor ELISA.
Results: Bisphenol A-HRP showed the highest detectability among six EDCs, with weak estrogen receptor activity and good spiked recovery (80-110%).
Conclusion: This strategy suggests that biomarkers with weak competitiveness can enhance detectability in competitive ELISA.
Keywords
Competitive ELISA; Estrogen disrupting chemicals; Estrogen receptor; Molecular docking; Weak competitive molecule.
