Affiliations
- Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui Medical University, Hefei, China.
- Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui Medical University, Hefei, China. xsqian-1112@163.com.
PMID: 37866658 DOI: 10.1016/j.ygeno.2023.110730
Abstract
Background: RNA-binding proteins (RBPs) are crucial for gene expression and immune regulation. This study explored the role of RBPs in ankylosing spondylitis (AS) and identified RIOK3 as a key RBP influencing disease pathogenesis.
Methods: RNA-seq was used to analyze PBMCs from five AS patients and three healthy individuals, identifying RIOK3 as a significant RBP. siRNA knockdown experiments in mBMSCs were performed to understand its impact on gene expression.
Results: RIOK3 knockdown inhibited osteogenic differentiation and ribosome function in mBMSCs, suggesting its involvement in pathological bone formation in AS.
Conclusion: RIOK3 may play a critical role in AS pathogenesis by influencing bone formation, making it a potential target for therapeutic intervention.
Keywords
Ankylosing spondylitis; Bioinformatics; Bone marrow mesenchymal stem cell; New bone formation; RNA-binding proteins.
