Affiliations
- National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
- Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan.
- College of Veterinary Medicine, Northwest Agricultural and Forestry University, Yangling, China.
- Hubei Engineering Research Center in Buffalo Breeding and Products, Wuhan, China.
PMID: 36891049 PMCID: PMC9986533 DOI: 10.3389/fendo.2023.1076404
Abstract
Introduction: Inhibin DNA vaccine has been shown to improve animal fertility. This study explores the effects of a novel Anti-Müllerian hormone (AMH)-Inhibin (INH)-RF-amide-related peptides (RFRP) DNA vaccine on immune response and reproductive performance in buffalo.
Methods: A total of 84 buffaloes were divided into four groups and immunized with different doses of AMH-INH-RFRP DNA vaccine or PBS, with boosters given after 14 days. Antibody titers, hormone levels, and reproductive parameters were analyzed.
Results: Higher antibody titers were observed in the group receiving a moderate vaccine dose. Increased E2 levels, ovulation rates, and conception rates were observed in antibody-positive buffaloes.
Conclusion: The novel AMH-INH-RFRP DNA vaccine enhances reproductive performance in buffalo by promoting E2 production and follicular growth.
Keywords
AMH; DNA vaccine; INH; RFRP; buffalo; reproduction.
