Affiliations
- Yangyang Zhang, Peking University First Hospital
- Yang Xu, Peking University First Hospital (xuyangm@126.com)
- Yanrong Kuai, Peking University First Hospital
- Sheng Wang, Peking University First Hospital
- Jiao Yu, Peking University First Hospital
Abstract
Background: Poor ovarian response (POR) is one of the most challenging issues in assisted reproductive technology. Given the importance of androgens in follicle recruitment, growth and development, domestic and foreign reproductive centers have added androgen as an adjuvant to in vitro fertilization protocols for patients with POR to improve the ovarian response. The mechanism underlying the effects of androgens is unclear. The aim of this research was to study the effects of testosterone on granulosa cells and the underlying mechanisms.
Methods: The human ovarian granulosa cell line KGN was treated with testosterone at various concentrations and an androgen receptor antagonist.
Keywords: androgen, testosterone, connexin 43, phosphorylation Cx43, PKC, poor ovarian response.
