Catalog No.
KVV13301
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Monkeypox Virus M1R Antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Monkeypox Virus M1R Antigen present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled Monkeypox Virus M1R antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Monkeypox Virus M1R Antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Monkeypox Virus M1R Antigen concentration in serum and plasma.
Target
M1R
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
7.81 - 500 pg/mL
Sensitivity
1.9 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (pg/mL)
|
230.2
|
58.9
|
14.2
|
256.3
|
69.5
|
18.8
|
|
Standard deviation
|
7.8
|
1.9
|
1.1
|
24.1
|
7.9
|
2.7
|
|
CV (%)
|
3.4
|
3.2
|
7.7
|
9.4
|
11.4
|
14.4
|
Recovery
85-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
Note
For Research Use Only.
BoHV-4-Based Vector Single Heterologous Antigen Delivery Protects STAT1(-/-) Mice from Monkeypoxvirus Lethal Challenge., PMID:26086739
Rational development of multicomponent mRNA vaccine candidates against mpox., PMID:36947428
Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity., PMID:37300753
Mpox virus mRNA-lipid nanoparticle vaccine candidates evoke antibody responses and drive protection against the Vaccinia virus challenge in mice., PMID:37429529
Duration of humoral immunity from smallpox vaccination and its cross-reaction with Mpox virus., PMID:37709783
mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge., PMID:37739969
Evaluation and comparison of immune responses induced by two Mpox mRNA vaccine candidates in mice., PMID:37800627
In silico development of a novel anti-mutation, multi-epitope mRNA vaccine against MPXV variants of emerging lineage and sub-lineages by using immunoinformatics approaches., PMID:38450722
Polyvalent mpox mRNA vaccines elicit robust immune responses and confer potent protection against vaccinia virus., PMID:38787725
Analysis of binding and authentic virus-neutralizing activities of immune sera induced by various monkeypox virus antigens., PMID:38829493
Systematic evaluation of the induction of efficient neutralizing antibodies by recombinant multicomponent subunit vaccines against monkeypox virus., PMID:39321566
Mpox global health emergency: Insights into the virus, immune responses, and advancements in vaccines PART II: Insights into the advancements in vaccines., PMID:39417768
Single-chain A35R-M1R-B6R trivalent mRNA vaccines protect mice against both mpox virus and vaccinia virus., PMID:39423738
Computational identification of monkeypox virus epitopes to generate a novel vaccine antigen against Mpox., PMID:39471737
Programmable Macrophage Vesicle Based Bionic Self-Adjuvanting Vaccine for Immunization against Monkeypox Virus., PMID:39513669
A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates., PMID:39639037
Immunoinformatics approach: Developing a multi-epitope vaccine with novel carriers targeting monkeypox virus., PMID:39679938
Long-Lasting Protection and Dose Optimization of MPXV Polyvalent Mpox mRNA Vaccines Against Lethal Vaccinia Virus Challenge in Mice., PMID:39726255
Rational mpox vaccine design: immunogenicity and protective effect of individual and multicomponent proteins in mice., PMID:40105863
Generation and characterization of neutralizing antibodies against M1R and B6R proteins of monkeypox virus., PMID:40164599
Immunological analysis of LC16m8 vaccine: preclinical and early clinical insights into mpox., PMID:40239465
A Pathogen-Mimicking Monkeypox Virus Nanovaccine Inspired by Assembly of Viral Antigens with β-Glucan and Dendrimer., PMID:40366370
Monkeypoxvirus infections. PMID: 11189729
[Twelve cases of monkeypox virus outbreak in Bangassou District (Central African Republic) in December 2015]. PMID: 27783372
[Comparative study of variola virus and monkeypox virus interferon-gamma-binding]. PMID: 16358743
Emergence of human monkeypox in west Africa. PMID: 31285141
The role of squirrels in sustaining monkeypox virus transmission. PMID: 2820094
Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging. PMID: 26517839
[What are we talking about? When Monkeypox virus infection inadvertently results in smallpox in French]. PMID: 35792844
Outbreaks of human monkeypox after cessation of smallpox vaccination. PMID: 22239910
The monkeypox virus outbreak: reflections from the frontlines. PMID: 14608307
Strengthening of Surveillance during Monkeypox Outbreak, Republic of the Congo, 2017. PMID: 29774865
Monkeypox virus evades antiviral CD4+ and CD8+ T cell responses by suppressing cognate T cell activation. PMID: 18796610
Monkeypox Disease Outbreak (2022): Epidemiology, Challenges, and the Way Forward. PMID: 35762024
Experimental infection of prairie dogs with monkeypox virus. PMID: 15829191
Epidemiologic and Ecologic Investigations of Monkeypox, Likouala Department, Republic of the Congo, 2017. PMID: 30666937
Nanopore sequencing of a monkeypox virus strain isolated from a pustular lesion in the Central African Republic. PMID: 35750759
Monkeypox virus infection of rhesus macaques induces massive expansion of natural killer cells but suppresses natural killer cell functions. PMID: 24147080
[Perspectives on recent monkeypox outbreak in non-endemic areas]. PMID: 35872578
Environmental Persistence of Monkeypox Virus on Surfaces in Household of Person with Travel-Associated Infection, Dallas, Texas, USA, 2021. PMID: 35951009
Evasion of the Innate Immune Type I Interferon System by Monkeypox Virus. PMID: 26246580
A case of imported Monkeypox in Singapore. PMID: 31657773
Proteomic basis of the antibody response to monkeypox virus infection examined in cynomolgus macaques and a comparison to human smallpox vaccination. PMID: 21209900
Monkeypox Rash Severity and Animal Exposures in the Democratic Republic of the Congo. PMID: 31875271
Monkeypox - Past as Prologue. PMID: 36001716
Monkeypox: a neglected old foe. PMID: 35691304
Monkeypox virus as a source of whitepox viruses. PMID: 91589
Monkeypox virus induces the synthesis of less dsRNA than vaccinia virus, and is more resistant to the anti-poxvirus drug, IBT, than vaccinia virus. PMID: 27467578
Characterizing monkeypox virus specific CD8+ T cell epitopes in rhesus macaques. PMID: 24210113
Lethal monkeypox virus infection of CAST/EiJ mice is associated with a deficient gamma interferon response. PMID: 22696658
Sequence of pathogenic events in cynomolgus macaques infected with aerosolized monkeypox virus. PMID: 25653439
Co-administration of tecovirimat and ACAM2000鈩?in non-human primates: Effect of tecovirimat treatment on ACAM2000 immunogenicity and efficacy versus lethal monkeypox virus challenge. PMID: 31677948
Recent advances in the diagnosis monkeypox: implications for public health. PMID: 35997157
Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus-infected cells reveals virus-specific regulation of immune response genes. PMID: 23425254
Factors affecting the likelihood of monkeypox's emergence and spread in the post-smallpox era. PMID: 22709519
Intranasal monkeypox marmoset model: Prophylactic antibody treatment provides benefit against severe monkeypox virus disease. PMID: 29927927
Attenuation of monkeypox virus by deletion of genomic regions. PMID: 25462353
Monkeypox virus and whitepox viruses. PMID: 35952
Comparison of Multiplexed Immunofluorescence Imaging to Chromogenic Immunohistochemistry of Skin Biomarkers in Response to Monkeypox Virus Infection. PMID: 32717786
A phylogeographic investigation of African monkeypox. PMID: 25912718
Possibility of vertical transmission of the human monkeypox virus. PMID: 35963577
Human monkeypox. PMID: 15451482
Guidelines for pregnant individuals with monkeypox virus exposure. PMID: 35750071
Monkeypox: another test for PCR. PMID: 35959687
Assessing Monkeypox Virus Prevalence in Small Mammals at the Human-Animal Interface in the Democratic Republic of the Congo. PMID: 28972544
Inhalational monkeypox virus infection in cynomolgus macaques. PMID: 23061051
Monkeypox virus: ecology and public health significance. PMID: 2853010
Cross-Neutralizing and Protective Human Antibody Specificities to Poxvirus Infections. PMID: 27768891
Human Monkey Pox Virus Infection in Plateau State, North Central Nigeria: A Report of Two Cases. PMID: 35038257
Importance of epidemiological research of monkeypox: is incidence increasing? PMID: 32096659
For research use only. Not for human or drug use.
