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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (M1R)

Catalog #:   KAV13301 Specific References (144) DATASHEET
Applications: Used for the quantitative determination of Human Anti-Monkeypox virus IgG (M1R) concentration in serum and plasma.
Sample type: Plasma, Serum
Sensitivity: 6.9 ng/mL
Range: 15.63 - 1,000 ng/mL
Overview

Catalog No.

KAV13301

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus M1R antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (M1R) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (M1R). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (M1R) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

M1R

Applications

Used for the quantitative determination of Human Anti-Monkeypox virus IgG (M1R) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

15.63 - 1,000 ng/mL

Sensitivity

6.9 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

 

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

16

16

16

24

24

24

Mean (ng/mL)

377.5

110.5

32.0

360.5

102.8

32.4

Standard deviation

13.3

4.7

4.3

21.4

3.7

3.3

CV (%)

3.5

4.2

13.6

5.9

3.6

10.1

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Data Image
  • Experiment Example
    CALCULATION OF RESULTS
    Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. Do not include the blank in the standard curve. The data may be linearized by plotting the log of the Human Anti-Monkeypox virus IgG (M1R) concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
References

Inhibition of monkeypox virus replication by RNA interference. PMID: 19889227

Monkeypox virus viral chemokine inhibitor (MPV vCCI), a potent inhibitor of rhesus macrophage inflammatory protein-1. PMID: 18639466

Susceptibility of Marmosets (Callithrix jacchus) to Monkeypox Virus: A Low Dose Prospective Model for Monkeypox and Smallpox Disease. PMID: 26147658

Comparative live bioluminescence imaging of monkeypox virus dissemination in a wild-derived inbred mouse (Mus musculus castaneus) and outbred African dormouse (Graphiurus kelleni). PMID: 25462355

Substrate specificity of homogeneous monkeypox virus uracil-DNA glycosylase. PMID: 17910420

The First Case of Monkeypox in the Republic of Korea. PMID: 35818706

Modified vaccinia virus Ankara protects macaques against respiratory challenge with monkeypox virus. PMID: 15919938

[Present status of monkeypox]. PMID: 10549024

Susceptibility of monkeypox virus aerosol suspensions in a rotating chamber. PMID: 23142251

[Development of the disease in marmot at the intranasal infection with the monkeypox virus]. PMID: 27024916

Monoclonal antibodies to a monkeypox virus polypeptide determinant. PMID: 6207310

The relevance of multiple clinical specimens in the diagnosis of monkeypox virus, Spain, June 2022. PMID: 35983771

The presumed receptivity and susceptibility to monkeypox of European animal species. PMID: 35753629

Geographic structuring and divergence time frame of monkeypox virus in the endemic region. PMID: 35831941

Transmissibility of the monkeypox virus clades via respiratory transmission: investigation using the prairie dog-monkeypox virus challenge system. PMID: 23408990

Genome Sequences of Akhmeta Virus, an Early Divergent Old World Orthopoxvirus. PMID: 29757202

Deletion of the monkeypox virus inhibitor of complement enzymes locus impacts the adaptive immune response to monkeypox virus in a nonhuman primate model of infection. PMID: 21752919

Finishing monkeypox genomes from short reads: assembly analysis and a neural network method. PMID: 27585810

Monkeypox: What do we know so far? A short narrative review of literature. PMID: 35833402

Monkeypox and its pandemic potential: what the anaesthetist should know. PMID: 35803753

Outbreaks of disease suspected of being due to human monkeypox virus infection in the Democratic Republic of Congo in 2001. PMID: 12149352

Laboratory studies on monkeypox virus. PMID: 6292283

Human monkeypox outbreak caused by novel virus belonging to Congo Basin clade, Sudan, 2005. PMID: 20875278

Diagnosis and assessment of monkeypox virus (MPXV) infection by quantitative PCR assay: differentiation of Congo Basin and West African MPXV strains. PMID: 18362406

LC16m8, a highly attenuated vaccinia virus vaccine lacking expression of the membrane protein B5R, protects monkeys from monkeypox. PMID: 16698998

Appearance and re-appearance of zoonotic disease during the pandemic period: long-term monitoring and analysis of zoonosis is crucial to confirm the animal origin of SARS-CoV-2 and monkeypox virus. PMID: 35658858

Cytokine modulation correlates with severity of monkeypox disease in humans. PMID: 25600603

Evaluation of monkeypox virus infection of black-tailed prairie dogs (Cynomys ludovicianus) using in vivo bioluminescent imaging. PMID: 24779460

Independent evolution of monkeypox and variola viruses. PMID: 1331540

New methylene blue derivatives suggest novel anti-orthopoxviral strategies. PMID: 33992710

Ecological niche and geographic distribution of human monkeypox in Africa. PMID: 17268575

Clonal vaccinia virus grown in cell culture fully protects monkeys from lethal monkeypox challenge. PMID: 18077063

Whitepox virus isolated from hamsters inoculated with monkeypox virus. PMID: 213730

How does monkeypox spread? What scientists know. PMID: 35953575

Monkeypox transmission and pathogenesis in prairie dogs. PMID: 15109408

Attenuated NYCBH vaccinia virus deleted for the E3L gene confers partial protection against lethal monkeypox virus disease in cynomolgus macaques. PMID: 22001879

Human monkeypox--Kasai Oriental, Zaire, 1996-1997. PMID: 9132583

Elucidating the role of the complement control protein in monkeypox pathogenicity. PMID: 22496894

Varicella Coinfection in Patients with Active Monkeypox in the Democratic Republic of the Congo. PMID: 28894977

The evolving epidemiology of viral encephalitis. PMID: 16914972

DNA/MVA HIV-1/AIDS vaccine elicits long-lived vaccinia virus-specific immunity and confers protection against a lethal monkeypox challenge. PMID: 17507071

Human monkeypox: secondary attack rates. PMID: 2844429

Nonhuman primates are protected from smallpox virus or monkeypox virus challenges by the antiviral drug ST-246. PMID: 19349521

A novel respiratory model of infection with monkeypox virus in cynomolgus macaques. PMID: 21389129

Enhancing Oligodendrocyte Myelination Rescues Synaptic Loss and Improves Functional Recovery after Chronic Hypoxia. PMID: 30078577

Evidence that anti-muscarinic antibodies in Sjögren's syndrome recognise both M3R and M1R. PMID: 18249005

Immunohistochemical localisation of cholinergic muscarinic receptor subtype 1 (M1r) in the guinea pig and human enteric nervous system. PMID: 17462859

123I-iododexetimide preferentially binds to the muscarinic receptor subtype M1 in vivo. PMID: 25593117

Localization of muscarinic receptors M1R, M2R and M3R in the human colon. PMID: 20146726

Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex. PMID: 29424434

M1 muscarinic receptors modify oxidative stress response to acetaminophen-induced acute liver injury. PMID: 25452146

M1 Muscarinic Receptor Deficiency Attenuates Azoxymethane-Induced Chronic Liver Injury in Mice. PMID: 26374068

Diverse glutamatergic inputs target spines expressing M1 muscarinic receptors in the basolateral amygdala: An ultrastructural analysis. PMID: 31348911

Muscarinic cholinergic receptor M1 in the rat basolateral amygdala: ultrastructural localization and synaptic relationships to cholinergic axons. PMID: 23559406

Neuronal localization of m1 muscarinic receptor immunoreactivity in the monkey basolateral amygdala. PMID: 33410202

Dynamic metabolic control of an ion channel. PMID: 24560147

M1 receptors interacting with NMDAR enhance delay-related neuronal firing and improve working memory performance. PMID: 35098156

Choices and Decisions in Decompressive Surgery for Thoracolumbar Intervertebral Disk Herniation. PMID: 29074335

PKC delta activation increases neonatal rat retinal cells survival in vitro: Involvement of neurotrophins and M1 muscarinic receptors. PMID: 29705702

Ovarian acetylcholine and muscarinic receptors: hints of a novel intrinsic ovarian regulatory system. PMID: 12467026

Treatment of bladder dysfunction with solifenacin: is there a risk of dementia or cognitive impairment? PMID: 35320336

M1-muscarinic receptors promote fear memory consolidation via phospholipase C and the M-current. PMID: 24478341

Ovulation requires the activation on proestrus of M₁ muscarinic receptors in the left ovary. PMID: 25586874

M1 acetylcholine receptors in somatostatin interneurons contribute to GABAergic and glutamatergic plasticity in the mPFC and antidepressant-like responses. PMID: 37142667

Muscarinic M1 receptor partially modulates higher sensitivity to cadmium-induced cell death in primary basal forebrain cholinergic neurons: A cholinesterase variants dependent mechanism. PMID: 27377441

StAR protein is increased by muscarinic receptor activation in human luteinized granulosa cells. PMID: 11165010

Potential Role for Combined Subtype-Selective Targeting of M1 and M3 Muscarinic Receptors in Gastrointestinal and Liver Diseases. PMID: 34803723

A self-limiting regulation of vasoconstrictor-activated TRPC3/C6/C7 channels coupled to PI(4,5)P₂-diacylglycerol signalling. PMID: 22183723

Corrigendum: Striatal dopamine D2-muscarinic acetylcholine M1 receptor-receptor interaction in a model of movement disorders. PMID: 36467044

Regulation of Kv2.1 channel inactivation by phosphatidylinositol 4,5-bisphosphate. PMID: 29379118

Rational development of multicomponent mRNA vaccine candidates against mpox. PMID: 36947428

Gi/o-coupled muscarinic receptors co-localize with GIRK channel for efficient channel activation. PMID: 30240440

Cadmium-induced neurotoxic effects on rat basal forebrain cholinergic system through thyroid hormones disruption. PMID: 34968718

Distinct microplastic patterns in the sediment and biota of an urban stream. PMID: 35660581

Prolonged Systemic Inflammation Alters Muscarinic Long-Term Potentiation (mLTP) in the Hippocampus. PMID: 33510779

Click Chemistry-Enabled Conjugation Strategy for Producing Dibenzodiazepinone-Type Fluorescent Probes To Target M2 Acetylcholine Receptors. PMID: 36327428

Analysis on interrelation between electroacupuncture-induced cumulative analgesic effect and hypothalamic cholinergic activities in chronic neuropathic pain rats. PMID: 22936324

Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning. PMID: 35665767

Conjugation of Short Peptides to Dibenzodiazepinone-Type Muscarinic Acetylcholine Receptor Ligands Determines M2R Selectivity. PMID: 31074983

Dorsal hippocampal muscarinic cholinergic receptors orchestrate behavioral and autonomic changes induced by contextual fear retrieval. PMID: 35798059

Destination and consequences of Panx1 and mutant expression in polarized MDCK cells. PMID: 31102595

Muscarinic Acetylcholine M2 Receptors Regulate Lateral Habenula Neuron Activity and Control Cocaine Seeking Behavior. PMID: 35764382

Behavioral effects of Bj-PRO-7a, a proline-rich oligopeptide from Bothrops jararaca venom. PMID: 31721904

Novel 3-(1,2,3,6-Tetrahydropyridin-4-yl)-1H-indole-Based Multifunctional Ligands with Antipsychotic-Like, Mood-Modulating, and Procognitive Activity. PMID: 28763213

Activation of Muscarinic M1 Acetylcholine Receptors Induces Long-Term Potentiation in the Hippocampus. PMID: 26472558

Activation of muscarinic acetylcholine receptor 1 promotes invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition. PMID: 32097138

Selective antagonism of muscarinic receptors is neuroprotective in peripheral neuropathy. PMID: 28094765

Maintenance of CaV2.2 channel-current by PIP2 unveiled by neomycin in sympathetic neurons of the rat. PMID: 31923392

Caffeic acid attenuates neuroinflammation and cognitive impairment in streptozotocin-induced diabetic rats: Pivotal role of the cholinergic and purinergic signaling pathways. PMID: 36796549

Type 1 melanocortin receptors in pro-opiomelanocortin-, vasopressin-, and oxytocin-immunopositive neurons in different areas of mouse brain. PMID: 35475324

Red-Emitting Dibenzodiazepinone Derivatives as Fluorescent Dualsteric Probes for the Muscarinic Acetylcholine M2 Receptor. PMID: 32233403

Distance-dependent accuracy in Le Fort I maxillary repositioning procedures. PMID: 28843967

Homology modeling of human muscarinic acetylcholine receptors. PMID: 24328076

Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes. PMID: 31073061

Immunogenicity of MVA-BN vaccine deployed as mpox prophylaxis: a prospective, single-centre, cohort study and analysis of transcriptomic predictors of response., PMID:40286799

The two-dose MVA-BN mpox vaccine induces a nondurable and low avidity MPXV-specific antibody response., PMID:40162783

Rational mpox vaccine design: immunogenicity and protective effect of individual and multicomponent proteins in mice., PMID:40105863

Development and validation of a quantitative Orthopoxvirus immunoassay to evaluate and differentiate serological responses to Mpox infection and vaccination., PMID:39987746

Short-term evolution of Mpox-specific IgG and neutralizing antibodies among individuals undergoing MVA-BN vaccination., PMID:39894441

Enhanced Immunogenicity and Affinity with A35R-Fc-Based Chimeric Protein Compared to MPXV A35R Protein., PMID:39861905

Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study., PMID:39832516

Short-Lived Neutralizing Antibody Responses to Monkeypox Virus in Smallpox Vaccine-Naive Persons after JYNNEOS Vaccination., PMID:39793541

An mpox quadrivalent mRNA vaccine elicits sustained and protective immunity in mice against lethal vaccinia virus challenge., PMID:39745170

Long-Lasting Protection and Dose Optimization of MPXV Polyvalent Mpox mRNA Vaccines Against Lethal Vaccinia Virus Challenge in Mice., PMID:39726255

Elderly Individuals Exhibit Elevated Levels of Anti-Monkeypox Virus Antibodies Compared to Adults, Youth, and Children., PMID:39569454

Unique Kinetics of the Human Milk Antibody Response to JYNNEOS Vaccine for Prevention of Monkey Pox: A Case Study., PMID:39360771

Rational design and computational evaluation of a multi-epitope vaccine for monkeypox virus: Insights into binding stability and immunological memory., PMID:39247273

Investigating seroprevalence of IgG antibodies against Monkeypox Virus (MPXV) in a cohort of people living with HIV (PLWH) in Rome, during the 2022 outbreak: Moving beyond traditional at-risk populations., PMID:39241937

An Attenuated and Highly Immunogenic Variant of the Vaccinia Virus., PMID:39188266

Profiling of viral load, antibody and inflammatory response of people with monkeypox during hospitalization: a prospective longitudinal cohort study in China., PMID:39043012

Clinical characteristics, viral dynamics, and antibody response of monkeypox virus infections among men with and without HIV infection in Guangzhou, China., PMID:38979508

Trimerized S expressed by modified vaccinia virus Ankara (MVA) confers superior protection against lethal SARS-CoV-2 challenge in mice., PMID:38874361

Immune responses associated with mpox viral clearance in men with and without HIV in Spain: a multisite, observational, prospective cohort study., PMID:38857615

Third-generation smallpox vaccines induce low-level cross-protecting neutralizing antibodies against Monkeypox virus in laboratory workers., PMID:38826712

Longitudinal viral shedding and antibody response characteristics of men with acute infection of monkeypox virus: a prospective cohort study., PMID:38802350

Antibodies Induced by Smallpox Vaccination after at Least 45 Years Cross-React with and In Vitro Neutralize Mpox Virus: A Role for Polyclonal B Cell Activation?, PMID:38675961

Scenarios of future mpox outbreaks among men who have sex with men: a modelling study based on cross-sectional seroprevalence data from the Netherlands, 2022., PMID:38666400

Mpox outbreak in Rio de Janeiro, Brazil: A translational approach., PMID:38654686

Breakthrough Mpox Outbreak Investigation, the Delicate Balance Between Host Immune Response and Viral Immune Escape., PMID:38647249

A multivalent mRNA monkeypox virus vaccine (BNT166) protects mice and macaques from orthopoxvirus disease., PMID:38366591

Characterizing the acute antibody response of monkeypox and MVA-BN vaccine following an Australian outbreak., PMID:38240403

Review of virological methods for laboratory diagnosis and characterization of monkeypox virus (MPXV): lessons learned from the 2022 Mpox outbreak., PMID:38179904

Serological methods for the detection of antibodies against monkeypox virus applicable for laboratories with different biosafety levels., PMID:38054557

Serosurvey of Immunity to Monkeypox (Mpox) Virus Antigens in People Living with HIV in South Florida., PMID:38003819

Smallpox vaccination in a mouse model., PMID:37965374

Prevalence of Undiagnosed Monkeypox Virus Infections during Global Mpox Outbreak, United States, June-September 2022., PMID:37832516

Mpox as AIDS-defining event with a severe and protracted course: clinical, immunological, and virological implications., PMID:37778364

Duration of humoral immunity from smallpox vaccination and its cross-reaction with Mpox virus., PMID:37709783

A Monoclonal Antibody Produced in Glycoengineered Plants Potently Neutralizes Monkeypox Virus., PMID:37514995

Mpox virus mRNA-lipid nanoparticle vaccine candidates evoke antibody responses and drive protection against the Vaccinia virus challenge in mice., PMID:37429529

Cross-reactive antibodies against monkeypox virus exist in the population immunized with vaccinia Tian Tan strain in China., PMID:37392823

Early evaluation of the safety, reactogenicity, and immune response after a single dose of modified vaccinia Ankara-Bavaria Nordic vaccine against mpox in children: a national outbreak response., PMID:37336224

Overview of Diagnostic Methods, Disease Prevalence and Transmission of Mpox (Formerly Monkeypox) in Humans and Animal Reservoirs., PMID:37317160

Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity., PMID:37300753

Profiling the acute phase antibody response against mpox virus in patients infected during the 2022 outbreak., PMID:37287343

Computational Vaccine Design for Poxviridae Family Viruses., PMID:37258933

Serological responses to smallpox A33 antigen and monkeypox A35 antigen in healthy people and people living with HIV-1 from Guangzhou, China., PMID:37212313

Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus., PMID:37117161

Proteomics-based vaccine targets annotation and design of subunit and mRNA-based vaccines for Monkeypox virus (MPXV) against the recent outbreak., PMID:37116237

Monkeypox (mpox) in immunosuppressed patients., PMID:37089133

Clinical characterization and placental pathology of mpox infection in hospitalized patients in the Democratic Republic of the Congo., PMID:37079637

Design and Optimization of a Monkeypox virus Specific Serological Assay., PMID:36986317

Kinetics of viral DNA in body fluids and antibody response in patients with acute Monkeypox virus infection., PMID:36748085

Comparison of the Effectiveness of Transepidemal and Intradermal Immunization of Mice with the Vacinia Virus., PMID:36694907

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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (M1R) [KAV13301]
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