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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (M1R) (KAV13301)

Applications:Used for the quantitative determination of Human Anti-Monkeypox virus IgG (M1R) concentration in serum and plasma.
Sample type:Plasma, Serum
Range:15.6 ng/mL - 1,000 ng/mL
Overview

Catalog No.

KAV13301

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus M1R antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (M1R) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (M1R). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (M1R) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

M1R

Applications

Used for the quantitative determination of Human Anti-Monkeypox virus IgG (M1R) concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

15.6 ng/mL - 1,000 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <20%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <20%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

 

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

16

16

16

24

24

24

Mean (ng/mL)

377.5

110.5

32.0

360.5

102.8

32.4

Standard deviation

13.3

4.7

4.3

21.4

3.7

3.3

CV (%)

3.5

4.2

13.6

5.9

3.6

10.1

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.

Data Image
  • Experiment Example
    CALCULATION OF RESULTS
    Average the duplicate readings for each standard and sample. Construct a standard curve by plotting the mean absorbance for each standard on the Y-axis against the concentration on the X-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Human Anti-Monkeypox virus IgG (M1R) concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
    If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
References

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LC16m8, a highly attenuated vaccinia virus vaccine lacking expression of the membrane protein B5R, protects monkeys from monkeypox. PMID: 16698998

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Cytokine modulation correlates with severity of monkeypox disease in humans. PMID: 25600603

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New methylene blue derivatives suggest novel anti-orthopoxviral strategies. PMID: 33992710

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Clonal vaccinia virus grown in cell culture fully protects monkeys from lethal monkeypox challenge. PMID: 18077063

Whitepox virus isolated from hamsters inoculated with monkeypox virus. PMID: 213730

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Monkeypox transmission and pathogenesis in prairie dogs. PMID: 15109408

Attenuated NYCBH vaccinia virus deleted for the E3L gene confers partial protection against lethal monkeypox virus disease in cynomolgus macaques. PMID: 22001879

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Elucidating the role of the complement control protein in monkeypox pathogenicity. PMID: 22496894

Varicella Coinfection in Patients with Active Monkeypox in the Democratic Republic of the Congo. PMID: 28894977

The evolving epidemiology of viral encephalitis. PMID: 16914972

DNA/MVA HIV-1/AIDS vaccine elicits long-lived vaccinia virus-specific immunity and confers protection against a lethal monkeypox challenge. PMID: 17507071

Human monkeypox: secondary attack rates. PMID: 2844429

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Enhancing Oligodendrocyte Myelination Rescues Synaptic Loss and Improves Functional Recovery after Chronic Hypoxia. PMID: 30078577

Evidence that anti-muscarinic antibodies in Sjögren's syndrome recognise both M3R and M1R. PMID: 18249005

Immunohistochemical localisation of cholinergic muscarinic receptor subtype 1 (M1r) in the guinea pig and human enteric nervous system. PMID: 17462859

123I-iododexetimide preferentially binds to the muscarinic receptor subtype M1 in vivo. PMID: 25593117

Localization of muscarinic receptors M1R, M2R and M3R in the human colon. PMID: 20146726

Immunolocalization of muscarinic M1 receptor in the rat medial prefrontal cortex. PMID: 29424434

M1 muscarinic receptors modify oxidative stress response to acetaminophen-induced acute liver injury. PMID: 25452146

M1 Muscarinic Receptor Deficiency Attenuates Azoxymethane-Induced Chronic Liver Injury in Mice. PMID: 26374068

Diverse glutamatergic inputs target spines expressing M1 muscarinic receptors in the basolateral amygdala: An ultrastructural analysis. PMID: 31348911

Muscarinic cholinergic receptor M1 in the rat basolateral amygdala: ultrastructural localization and synaptic relationships to cholinergic axons. PMID: 23559406

Neuronal localization of m1 muscarinic receptor immunoreactivity in the monkey basolateral amygdala. PMID: 33410202

Dynamic metabolic control of an ion channel. PMID: 24560147

M1 receptors interacting with NMDAR enhance delay-related neuronal firing and improve working memory performance. PMID: 35098156

Choices and Decisions in Decompressive Surgery for Thoracolumbar Intervertebral Disk Herniation. PMID: 29074335

PKC delta activation increases neonatal rat retinal cells survival in vitro: Involvement of neurotrophins and M1 muscarinic receptors. PMID: 29705702

Ovarian acetylcholine and muscarinic receptors: hints of a novel intrinsic ovarian regulatory system. PMID: 12467026

Treatment of bladder dysfunction with solifenacin: is there a risk of dementia or cognitive impairment? PMID: 35320336

M1-muscarinic receptors promote fear memory consolidation via phospholipase C and the M-current. PMID: 24478341

Ovulation requires the activation on proestrus of M₁ muscarinic receptors in the left ovary. PMID: 25586874

M1 acetylcholine receptors in somatostatin interneurons contribute to GABAergic and glutamatergic plasticity in the mPFC and antidepressant-like responses. PMID: 37142667

Muscarinic M1 receptor partially modulates higher sensitivity to cadmium-induced cell death in primary basal forebrain cholinergic neurons: A cholinesterase variants dependent mechanism. PMID: 27377441

StAR protein is increased by muscarinic receptor activation in human luteinized granulosa cells. PMID: 11165010

Potential Role for Combined Subtype-Selective Targeting of M1 and M3 Muscarinic Receptors in Gastrointestinal and Liver Diseases. PMID: 34803723

A self-limiting regulation of vasoconstrictor-activated TRPC3/C6/C7 channels coupled to PI(4,5)P₂-diacylglycerol signalling. PMID: 22183723

Corrigendum: Striatal dopamine D2-muscarinic acetylcholine M1 receptor-receptor interaction in a model of movement disorders. PMID: 36467044

Regulation of Kv2.1 channel inactivation by phosphatidylinositol 4,5-bisphosphate. PMID: 29379118

Rational development of multicomponent mRNA vaccine candidates against mpox. PMID: 36947428

Gi/o-coupled muscarinic receptors co-localize with GIRK channel for efficient channel activation. PMID: 30240440

Cadmium-induced neurotoxic effects on rat basal forebrain cholinergic system through thyroid hormones disruption. PMID: 34968718

Distinct microplastic patterns in the sediment and biota of an urban stream. PMID: 35660581

Prolonged Systemic Inflammation Alters Muscarinic Long-Term Potentiation (mLTP) in the Hippocampus. PMID: 33510779

Click Chemistry-Enabled Conjugation Strategy for Producing Dibenzodiazepinone-Type Fluorescent Probes To Target M2 Acetylcholine Receptors. PMID: 36327428

Analysis on interrelation between electroacupuncture-induced cumulative analgesic effect and hypothalamic cholinergic activities in chronic neuropathic pain rats. PMID: 22936324

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Dorsal hippocampal muscarinic cholinergic receptors orchestrate behavioral and autonomic changes induced by contextual fear retrieval. PMID: 35798059

Destination and consequences of Panx1 and mutant expression in polarized MDCK cells. PMID: 31102595

Muscarinic Acetylcholine M2 Receptors Regulate Lateral Habenula Neuron Activity and Control Cocaine Seeking Behavior. PMID: 35764382

Behavioral effects of Bj-PRO-7a, a proline-rich oligopeptide from Bothrops jararaca venom. PMID: 31721904

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Activation of Muscarinic M1 Acetylcholine Receptors Induces Long-Term Potentiation in the Hippocampus. PMID: 26472558

Activation of muscarinic acetylcholine receptor 1 promotes invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition. PMID: 32097138

Selective antagonism of muscarinic receptors is neuroprotective in peripheral neuropathy. PMID: 28094765

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Datasheet
$ 1400
Product specifications
96T 1400

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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (M1R) [KAV13301]
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