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Flow Cytometry Antibodies
2022-11-01 257

Flow Cytometry uses fluorescent markers on antibodies or other proteins in order to quantifiably detect changes in protein expression via excitation of the various fluorescent markers. Flow cytometry can be used for detecting numerous targets either on the surface or within cells in the same sample.Flow Cytometry Antibodies allow scientists to detect a specific antigen, making them useful for characterizing the proteins on the surface of live cells. These antibodies can be directly labeled with a fluorescent marker or can be visualized by binding to a secondary antibody that is labeled with a fluorescent marker. This allows flow cytometry to sort cells based on more than one color, each representing a different antigen that is bound by a different antibody.

 

T cells
Target Clone number CAT. Species
CD3 SPV-T3a FHC27740 Human
OKT3 FHC27730 Human
UCHT-1 FHC27720 Human
145-2C11 FMC27740 Mouse
CD4 GK1.5 FHB95920 Human
Hu5A8 FMB95910 Mouse
CD7 3A1e FHC41820 Human
CD8 G10-1 FHK18510 Human
OKT8 FMB96030 Mouse
CD25 ADCT-301 FHB95820 Human
CD28 15E8# FHC83420 Human
CD45RO UCHL1 FHC34020 Human
CD62L HuDreg-55 FHD03310 Human
CD137 LOB12.3 FMG11010 Mouse
CD154 hu5c8 FHD85410 Human
CD279(PD-1) ANB-011 FHH02230 Human
J43 FMH02210 Mouse
CD223 LAG-525 FHD30410 Human
CD357(GITR) DTA-1 FMJ89810 Mouse
TIGIT SAA0153 FHH72410 Human
TCR beta SAA0174 FMK13810 Mouse
       
B cells
Target Clone number CAT. Species
CD5 5D7 FHC18820 Human
H65 FHC18810 Human
CD19 1D3 FHD10830 Human
4G7 FMD10810 Mouse
CD20 18B12 FMC90710 Mouse
CD21 HB5 FHD36810 Human
7G6 FMD36810 Mouse
CD24 3B6 FHD65220 Human
CD40 FGK45 FMD68910 Mouse
CD69 SAA0031 FHG11610 Human
CD72 W004 FHD48310 Human
CD80(B7-1) IDEC-114 FHE03410 Human
16-10A1 FME03410 Mouse
CD86 PMP2B10 FHE33810 Human
GL1 FME33810 Mouse
       
Dendritic cells
Target Clone number CAT. Species
CD1a 2113 FHC18710 Human
CD11b M1/70 FMC86630 Mouse
CD11C N418 FMD41110 Mouse
CD80(B7-1) IDEC-114 FHE03410 Human
16-10A1 FME03410 Mouse
CD83 60B10 FHF85910 Human
CD86 GL1 FME33810 Mouse
HLA-DRA 1D09C3 FHM00810 Human
CD11b M1/70 FMC86630 Mouse
CD11C N418 FMD41110 Mouse
CD8a 53-6.72 FMB96060 Mouse
CD80(B7-1) 16-10A1 FME03410 Mouse
CD86 GL1 FME33810 Mouse
CD103 8D5 FHE22910 Human
 
NK cells
Target Clone number CAT. Species
CD16 3G8 FHC35010 Human
CD16/CD32 2.4G2 FMK22710 Mouse
CD49b SAA0018 FHD23410 Human
NK1.1(CD161) KM12.4.7 FHG33810 Human
PK13 FMG33810 Mouse
TIGIT SAA0153 FHH72410 Human
CD56 huN901-DM1 FHC98510 Human
CD161 KM12.4.7 FHG33810 Human
PK136 FMG33810 Mouse
CD314 SAA0098 FHD72110 Human
       
Mesenchymai stem cells
Target Clone number CAT. Species
CD29 K20 FHC17610 Human
CD44 VFF-18 FHD14810 Human
CD54 SAA0025 FHC16010 Human
SAA0026 FHC16020 Human
CD73 TY/23 FMD46620 Human
CD90.2 30-H12 FMC07810 Mouse
CD106 H6 FHD33110 Human
CD105 SAA0041 FHD26510 Human
       
Megakaryocyte cells
Target Clone number CAT. Species
CD11b M1/70 FMC86630 Mouse
CD11C N418 FMD41110 Mouse
CD14 28C5 FHC33710 Human
CD16 3G8 FHC35010 Human
CD16/CD32 2.4G2 FMK22710 Mouse
CD80(B7-1) IDEC-114 FHE03410 Human
GL1 FME33810 Mouse
CD86 GL1 FME33810 Mouse
CD115(c-fms) AFS98 FMC25210 Mouse
CD163 M130 FHJ16310 Human
Ly-6G(Gr-1) 1A8 FMK18410 Mouse
       
Haematopoietic stem cells
Target Clone number CAT. Species
CD34 QBEnd-10 FHD80720 Human
CD38 38SB19 FHD80820 Human
TAK-079 FHD80830 Human
CD117 SAA0043 FHC83110 Human
       
       
Isotype control
Species Isotype Clone number CAT.
Rat IgG1 HRPH FRJ92860
IgG2a 2A3 FRB96740
IgG2b LTF-2 FRB96810
IgM, kappa RTK2118 FRJ93220
Mouse IgG1 MOPC-21 FMJ92820
IgG2a C1.18.4 FMB96720
IgG2b MPC-11 FMB96820
IgM IS5-20C4 FMJ93210
Human IgG1 REA293 FHJ92820
IgM SPE-7 FHJ93220

 

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