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Anti-Twin-Strep-tag Antibody (SAA0348)

Catalog #:   RGK26103 Specific References (7) DATASHEET
Host species: Mouse
Isotype: IgG2b
Applications: ELISA, WB
Overview

Catalog No.

RGK26103

Species reactivity

General

Host species

Mouse

Isotype

IgG2b

Clonality

Monoclonal

Tested applications

ELISA: 1:4000-1:8000, WB: 1:1000-1:5000

Target

Twin-Strep-tag, Twin Strep tag, TwinStrep tag,Streptag,Strep tag, SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Concentration

4.58 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

ELISA, WB

Form

Liquid

Storage buffer

100 mM Pro-Ac, 20 mM Arg, pH 5.0

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0348

Data Image
  • Western Blot
    Various Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with Twin-Strep-tag antibody (RGK26103) at 1μg/ml.

    Lane 1: Recombinant Protein 1-Strep Tag
    Lane 2: Recombinant Protein 2-Strep Tag
    Lane 3: Recombinant Protein 3-Strep Tag

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

References

Recombinantly Expressed Tagged SUrface Protein (RETSUP) assay: a new diagnostic system for the detection of antibodies to platelets., PMID:38184205

RNA and Protein Interactomes of an RNA-Binding Protein Tagged with FLAG Epitopes Using Combinatory Approaches of Genome Engineering and Stable Transfection., PMID:37166670

THETA system allows one-step isolation of tagged proteins through temperature-dependent protein-peptide interaction., PMID:31240245

Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain., PMID:29143175

Programmable polyproteams built using twin peptide superglues., PMID:26787909

Organophosphate hydrolase in Brevundimonas diminuta is targeted to the periplasmic face of the inner membrane by the twin arginine translocation pathway., PMID:19700527

Sperm ubiquitination in patients with dysplasia of the fibrous sheath., PMID:12151447

Datasheet
$ 130
Product specifications
50 μg 130 100 μg 220

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Twin-Strep-tag Antibody (SAA0348) [RGK26103]
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