InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001) (VHB94601)

specific references (0) DATASHEET

Host species：Human

Isotype：IgG1

Applications：ELISA, FCM, Neutralization

Accession：P01563

Overview

Catalog No.

VHB94601

Species reactivity

Human

Host species

Human

Isotype

IgG1

Clonality

Monoclonal

Target

Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2,IFN-Alpha, IFNA2A, IFNA2B, IFNA2C, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFNA1, IFNA13, IFN-α4, IFN-α5, IFN-α6, IFN-α7, IFN-α8, IFN-αl0, IFN-α14, IFN-α16, IFN-α17, IFN-α21

Concentration

3.53 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01563

Applications

ELISA, FCM, Neutralization

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Iv0001

Data Image

Bioactivity

Detects Human IFN α2/IFNA2 in indirect ELISAs.
Flow-cytometry

Flow-cytometry using anti-human IFN α2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # VHB94601 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IFN α2 antibody.SHSY-5Y cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IFN α2 antibody monoclonal antibody (Catalog # VHB94601 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Western Blot

Various lysates were subjected to SDS PAGE followed by western blot with IFNA2 antibody (VHB94601) at 1 µg/ml.

Lane 1: IFNA2 transfected HEK293 cell lysate
Lane 2: Non-transfected HEK293 cell lysate

Second Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 μg/mL.

Predict MW: 21 kDa
Observed MW: 21 kDa

References

Datasheet

Document Download

InVivoMAb Anti-Human IFN α2/IFNA2 (Iv0001).pdf

$ 372

Product specifications

100 μg 372 1 mg 1486

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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