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Anti-TOMM70 Polyclonal Antibody (PHB45101)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:O94826
Overview

Catalog No.

PHB45101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TOMM70 (Leu333-Leu608).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

TOM70,Translocase of outer membrane 70 kDa subunit,Mitochondrial import receptor subunit TOM70,Mitochondrial precursor proteins import receptor,KIAA0719,TOMM70A,TOMM70,Translocase of outer mitochondrial membrane protein 70

Purification

Purified by antigen affinity column.

Accession

O94826

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with TOMM70 antibody (PHB45101) at 0.58μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: HepG2 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 67 kDa
    Observed MW: 70 kDa
  • Immunocytochemistry/ Immunofluorescence
    TOMM70 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with TOMM70 (PHB45101) at 11.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TOMM70 antibody (PHB45101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-TOMM70 Polyclonal Antibody [PHB45101]
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