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Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1) (RHF46101)

Host species:Mouse
Isotype:IgG2b-kappa
Applications:ELISA, FCM, WB
Accession:P61769
Overview

Catalog No.

RHF46101

Species reactivity

Human

Host species

Mouse

Isotype

IgG2b-kappa

Clonality

Monoclonal

Target

Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3, B2M, CDABP0092, HDCMA22P

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P61769

Applications

ELISA, FCM, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

BBM.1

Data Image
  • Bioactivity
    Detects B2M/Beta-2-microglobulin in indirect ELISAs.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human B2M antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human B2M antibody monoclonal antibody (Catalog # RHF46101 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
References
Datasheet
$ 328
Product specifications
100 μg 328 1 mg 1640

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human B2M/Beta-2-microglobulin Antibody (BBM.1) [RHF46101]
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