Catalog No.
KAV12701
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus H3L antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (H3L) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (H3L). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (H3L) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Target
H3L
Applications
Used for the quantitative determination of Human Anti-Monkeypox virus IgG (H3L) concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
469 ng/mL - 30,000 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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|
n
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16
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16
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16
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24
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24
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24
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|
Mean (ng/mL)
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14016.4
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3933.7
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1003.5
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14800.0
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3916.3
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927.8
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|
Standard deviation
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1272.4
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372.6
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103.8
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1197.0
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417.3
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93.1
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|
CV (%)
|
9.1
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9.5
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10.3
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8.1
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10.7
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10.0
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Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
New p35 (H3L) Epitope Involved in Vaccinia Virus Neutralization and Its Deimmunization. PMID: 35746695
Vaccinia virus H3L envelope protein is a major target of neutralizing antibodies in humans and elicits protection against lethal challenge in mice. PMID: 16140750
Poly[bis-[μ(2)-(dimethyl-aza-nium-yl)methyl-enediphospho-nato]magnesium]. PMID: 21522287
Vaccinia virus envelope H3L protein binds to cell surface heparan sulfate and is important for intracellular mature virion morphogenesis and virus infection in vitro and in vivo. PMID: 10708453
Cell population data: much more to explore. PMID: 36622868
Characterization of the vaccinia virus H3L envelope protein: topology and posttranslational membrane insertion via the C-terminal hydrophobic tail. PMID: 10906204
Effects of deletion or stringent repression of the H3L envelope gene on vaccinia virus replication. PMID: 10906205
Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens. PMID: 36687315
Molecular Modelling and Insilico Engineering of PapMV-CP Towards Display and Development of Capripox Viral Like Particles Based on Immunogenic P32 Envelop Protein is the Homologous of the Vaccinia-Viral H3L Gene: An Insilico Approach. PMID: 32421016
Phylogenetic analysis of three genes of Penguinpox virus corresponding to Vaccinia virus G8R (VLTF-1), A3L (P4b) and H3L reveals that it is most closely related to Turkeypox virus, Ostrichpox virus and Pigeonpox virus. PMID: 19426497
Synthesis, structure, and phosphatase-like activity of a new trinuclear Gd complex with the unsymmetrical ligand H3L as a model for nucleases. PMID: 20170188
High spin d5 complexes of tris(6-hydroxymethyl-2-pyridylmethyl)amine (H3L): hepta-coordinated [Mn(H3L)]Cl2 and linear trinuclear [Fe3L2](ClO4)3. PMID: 18185873
4-. PMID: 10508341
Experimental charge-density study on the nickel(II) coordination complex [Ni(H3L)][NO3][PF6] [H3L = N,N',N''-tris(2-hydroxy-3-methylbutyl)-1,4,7-triazacyclononane]: a reappraisal. PMID: 16552157
Hexaaqua-manganese(II) bis-{[N-(3-meth-oxy-2-oxidobenzyl-idene)glycylglycinato]copper(II)} hexa-hydrate. PMID: 21579016
Evolution of the orthopoxvirus core genome. PMID: 36280003
A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. PMID: 10485266
A variety of spin-crossover behaviors depending on the counter anion: two-dimensional complexes constructed by NH...Cl- hydrogen bonds, [FeIIH3LMe]Cl.X (X = PF6 -, AsF6 -, SbF6 -, CF3SO3 -; H3L(Me) = Tris[2-{[(2-methylimidazol-4-yl)methylidene]amino}ethyl]amine). PMID: 16575927
Binding and removal of sulfate, phosphate, arsenate, tetrachloromercurate, and chromate in aqueous solution by means of an activated carbon functionalized with a pyrimidine-based anion receptor (HL). Crystal structures of [H3L(HgCl4)]·H2O and [H3L(HgBr4)]·H2O showing anion-π interactions. PMID: 20836504
Nature of copper(II)-lanthanide(III) magnetic interactions and generation of a large magnetic moment with magnetic anisotropy of 3d-4f cyclic cylindrical tetranuclear complexes [CuIILLnIII(hfac)2]2, (H3L = 1-(2-hydroxybenzamido)-2-(2-hydroxy-3-methoxybenzylideneamino)ethane and Hhfac = hexafluoroacetylacetone, LnIII = Eu, Gd, Tb, Dy). PMID: 12693220
Observing an analyzer's operational life cycle: a useful management tool for clinical laboratories. PMID: 30099409
Tunable Optical Molecular Thermometers Based on Metallacrowns. PMID: 36173924
Lanthanide cryptate monometallic coordination complexes. PMID: 32955062
Synthesis, characterization and antitubercular activities of heterocyclic selenosemicarbazones. PMID: 35661528
A new family of "H3L-like" histone genes. PMID: 15638457
Interlayer interaction of two-dimensional layered spin crossover complexes [FeIIH3L(Me)][FeIIL(Me)]X (X-=ClO4-, BF4-, PF6-, AsF6-, and SbF6-; H3L(Me)=tris[2-(((2-methylimidazol-4-yl)methylidene)amino)ethyl]amine). PMID: 14658894
Electronic structures and selective fluoride sensing features of Os(bpy)2(HL(2-)) and [{Os(bpy)2}2(μ-HL(2-))](2+) (H3L: 5-(1H-benzo[d]imidazol-2-yl)-1H-imidazole-4-carboxylic acid). PMID: 25112369
Design of pure heterodinuclear lanthanoid cryptate complexes. PMID: 34123326
ELISA for Molluscum Contagiosum Virus. PMID: 29120484
Antibacterial Activity of Two Zn-MOFs Containing a Tricarboxylate Linker. PMID: 36500760
Basophil counting in hematology analyzers: time to discontinue? PMID: 33554563
Isolation of a (dinitrogen)tricopper(I) complex. PMID: 25238198
Single-Molecule-Magnet FeII4FeIII2 and Antiferromagnetic FeIII4 Coordination Clusters. PMID: 31136160
A self-healable metallohydrogel for drug encapsulations and drug release. PMID: 37223407
Stepwise Coordination Assembly Approach toward Aluminum-Lanthanide-based Compounds. PMID: 32883079
Synthesis and magnetic properties of heterometal cyclic tetranuclear complexes [Cu(II)LM(II)(hfac)]2 (M(II) = Zn, Cu, Ni, Co, Fe, Mn; H3L = 1-(2-hydroxybenzamido)-2-((2-hydroxy-3-methoxybenzylidene)amino)ethane; Hhfac = hexafluoroacetylacetone). PMID: 12950197
An unusual open cubane structure in a mu(1,1)-azido- and alkoxo-bridged tetranuclear copper(II) complex, [Cu4L2(mu(1,1)-N3)2].5H2O (H3L = N,N'-(2-Hydroxylpropane-1,3-diyl)bis-salicylideneimine). PMID: 15476386
Solvent-Vapor-Induced Reversible Single-Crystal-to-Single-Crystal Transformation of a Triphosphaazatriangulene-Based Metal-Organic Framework. PMID: 31773880
Zn(II) coordination polymers: therapeutic activity and nursing application values against coronary heart disease. PMID: 35711619
Template Synthesis of Three-Dimensional Hexakisimidazolium Cages. PMID: 29394472
Biotin-conjugated tumour-targeting photocytotoxic iron(III) complexes. PMID: 23776297
Probing and pressing surfaces of hepatitis C virus-like particles. PMID: 30897421
Different Phenomena in Magnetic/Electrical Properties of Co(II) and Ni(II) Isomorphous MOFs. PMID: 33842790
Three-in-One Structural-Building-Mode-Based Ti16-Type Titanium Oxo Cluster Entirely Protected by the Ligands Benzoate and Salicylhydroxamate. PMID: 35639458
Two Mn(II) and Zn(II) Coordination Polymers: Aqueous-Phase Detection of Nitroaromatic Explosives and Protective Effect on Atherosclerosis. PMID: 35015178
Crystal transformation in Mn(II) metal-organic frameworks based on a one-dimensional chain precursor. PMID: 34152335
Dodecanuclear [Cu(II)6Gd(III)6] nanoclusters as magnetic refrigerants. PMID: 22409581
Modulating the Inclusive and Coordinating Ability of Thiacalix[4]arene and Its Antenna Effect on Yb3-Luminescence via Upper-Rim Substitution. PMID: 36296384
Self-Assembly of Antiferromagnetically-Coupled Copper(II) Supramolecular Architectures with Diverse Structural Complexities. PMID: 33256163
Charge Neutral [Cu2L2] and [Pd2L2] Metallocycles: Self-Assembly, Aggregation, and Catalysis. PMID: 34114797
Metal-Ion Metathesis and Properties of Triarylboron-Functionalized Metal-Organic Frameworks. PMID: 25929722
From Hydrogen Bond to van der Waals Force: Molecular Scalpel Strategy to Exfoliate a Two-Dimensional Metal-Organic Nanosheet. PMID: 35354284
Four new lanthanide-organic frameworks: selective luminescent sensing and magnetic properties. PMID: 27464998
Vanadium complexes with multidentate amine bisphenols. PMID: 25117739
For research use only. Not for human or drug use.
