Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus A29L antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (A29L) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (A29L). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (A29L) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Catalog No.
KAV13401
Applications
Used for the quantitative determination of Human Anti-Monkeypox virus IgG (A29L) concentration in serum and plasma.
Target
A29L
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.
Detection method
Colorimetric
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
16282.8 |
4176.8 |
1072.5 |
16037.3 |
4253.0 |
1036.4 |
|
Standard deviation |
1668.9 |
471.1 |
143.4 |
1661.9 |
466.5 |
213.8 |
|
CV (%) |
10.2 |
11.3 |
13.4 |
10.4 |
11.0 |
20.6 |
Sample type
Plasma, Serum
Assay type
Quantitative
Range
469 ng/mL - 30,000 ng/mL
Recovery
75-120%
Alternative Names
A29L, Monkeypox virus/MPXV,A29
Shipping
2-8 ℃
Note
For Research Use Only.
For research use only. Not for human or drug use.
