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Anti-Human PIEZO1 Polyclonal Antibody

Catalog #:   PHK29101 Specific References (23) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q92508
Overview

Catalog No.

PHK29101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PIEZO1 (Gln2206-Lys2426).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Piezo-type mechanosensitive ion channel component 1, FAM38A, PIEZO1, Protein FAM38A, KIAA0233, Mib, Membrane protein induced by beta-amyloid treatment

Purification

Purified by antigen affinity column.

Accession

Q92508

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PIEZO1 antibody (PHK29101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with PIEZO1 antibody (PHK29101) at 1 μg/ml.

    Lane 1: MCF-7

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 286 kDa
    Observed MW: 300 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat brain stained for PIEZO1 with PHK29101.
References

Blended Phenotypes From a SERPINA 11 Pathogenic Variant Over Underlying Immune Fetal Hydrops: A Rare Case Report and Literature Review., PMID:40406169

Identification of putative baroreceptors in human aortic arch by histological and omics analyses., PMID:40335722

Remote Activation of Mechanotransduction via Integrin Alpha-5 via Aptamer-Conjugated Magnetic Nanoparticles Promotes Osteogenesis., PMID:38258032

Piezo1-Mediated Neurogenic Inflammatory Cascade Exacerbates Ventricular Remodeling After Myocardial Infarction., PMID:38235590

Differential expression of PIEZO1 and PIEZO2 mechanosensitive channels in ocular tissues implicates diverse functional roles., PMID:37820892

Elucidating Mechanotransduction Processes During Magnetomechanical Neuromodulation Mediated by Magnetic Nanodiscs., PMID:37811002

PIEZO1 promotes ATP release from periodontal ligament cells following compression force., PMID:37632763

Increased Piezo1 expression in myofibroblasts in patients with symptomatic carotid atherosclerotic plaques undergoing carotid endarterectomy: A pilot study., PMID:37499697

Peripheral sensory neurons and non-neuronal cells express functional Piezo1 channels., PMID:37247618

Functional Expression of Mechanosensitive Piezo1/TRPV4 Channels in Mouse Osteoblasts., PMID:36792153

Piezo1-mediated mechanotransduction promotes entheseal pathological new bone formation in ankylosing spondylitis., PMID:36543525

Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration., PMID:35619555

High-throughput membrane-anchored proteome screening reveals PIEZO1 as a promising antibody-drug target for human esophageal squamous cell carcinoma., PMID:35608274

A Piezo1/KLF15/IL-6 axis mediates immobilization-induced muscle atrophy., PMID:35290243

Fluid shear stress enhances T cell activation through Piezo1., PMID:35260156

Seven technologies to watch in 2021., PMID:33500572

Inside Out Integrin Activation Mediated by PIEZO1 Signaling in Erythroblasts., PMID:32848880

Transcatheter Aortic Valve Implantation Represents an Anti-Inflammatory Therapy Via Reduction of Shear Stress-Induced, Piezo-1-Mediated Monocyte Activation., PMID:32697107

Piezo1 is required for outflow tract and aortic valve development., PMID:32251670

Mechanosensing by Peyer's patch stroma regulates lymphocyte migration and mucosal antibody responses., PMID:31611698

The Function of the Novel Mechanical Activated Ion Channel Piezo1 in the Human Osteosarcoma Cells., PMID:29065102

Small quantum dots conjugated to nanobodies as immunofluorescence probes for nanometric microscopy., PMID:25397889

Mechanotransduction channels of the trabecular meshwork., PMID:24215462

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human PIEZO1 Polyclonal Antibody [PHK29101]
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