Overview
Catalog No.
PHA51101
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human BCAT2 (Ala107-Val392).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
BCT2,BCAT2,BCAT(m),BCATM,Placental protein 18,Branched-chain-amino-acid aminotransferase,mitochondrial,PP18,ECA40
Purification
Purified by antigen affinity column.
Accession
O15382
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Data Image
-
Various lysates were subjected to SDS PAGE followed by western blot with BCAT2 antibody (PHA51101) at 0.56μg/ml.
Lane 1: Raji cell lysate
Lane 2: HepG2 cell lysate
Lane 3: 293T cell lysate
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 44 kDa
Observed MW: 40 kDa
-
Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with BCAT2 antibody (PHA51101) at 1 µg/ml.
Lane 1: Recombinant Protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 35 kDa
Observed MW: 35 kDa
References
Identification of the mitochondrial branched chain aminotransferase as a branched chain alpha-keto acid transport protein., PMID:8428987
BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity., PMID:36642843
Disrupted cardiac fibroblast BCAA catabolism contributes to diabetic cardiomyopathy via a periostin/NAP1L2/SIRT3 axis., PMID:37993768
Identification of New Single Nucleotide Polymorphisms Potentially Related to Small Ruminant Lentivirus Infection Susceptibility in Goats Based on Data Selected from High-Throughput Sequencing., PMID:39452702
Notch1 blockade by a novel, selective anti-Notch1 neutralizing antibody improves immunotherapy efficacy in melanoma by promoting an inflamed TME., PMID:39491031
Datasheet