Anti-HDAC3 Polyclonal Antibody

Catalog No.

PHA50901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HDAC3 (Met1-Gln376).

Applications

ELISA, IHC, WB

Recommended Dilution

Application	Dilution
ELISA	1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HD3,SMAP45,RPD3-2,Histone deacetylase 3,HDAC3

Purification

Purified by antigen affinity column.

Accession

O15379

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

WESTERN BLOT

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HDAC3 antibody (PHA50901) at 1 μg/ml.

Lane 1: Recombinant protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 45 kDa
Observed MW: 45 kDa
Immunocytochemistry/ Immunofluorescence

HDAC3 in HaCat Cell Line.
The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with HDAC3 (PHA50901) at 8.4 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).

Sodium butyrate stimulates expression of fibroblast growth factor 21 in liver by inhibition of histone deacetylase 3., PMID:22338096

DNA sequence-dependent compartmentalization and silencing of chromatin at the nuclear lamina., PMID:22726435

Aurora B is regulated by acetylation/deacetylation during mitosis in prostate cancer cells., PMID:22751009

Identifying acetylated proteins in mitosis., PMID:22903717

HDAC3-selective inhibitor enhances extinction of cocaine-seeking behavior in a persistent manner., PMID:23297220

Histone deacetylase inhibition attenuates transcriptional activity of mineralocorticoid receptor through its acetylation and prevents development of hypertension., PMID:23421989

Downregulation of the activating NKp30 ligand B7-H6 by HDAC inhibitors impairs tumor cell recognition by NK cells., PMID:23801635

Histone deacetylase 3 unconventional splicing mediates endothelial-to-mesenchymal transition through transforming growth factor β2., PMID:24045946

The corepressor Atrophin specifies odorant receptor expression in Drosophila., PMID:24334704

Combination of S-adenosylhomocysteine and scriptaid, a non-toxic epigenetic modifying reagent, modulates the reprogramming of bovine somatic-cell nuclear transfer embryos., PMID:24347442

The Notch co-repressor protein NKAP is highly expressed in adult mouse subventricular zone neural progenitor cells., PMID:24583034

Valproic acid prevents penile fibrosis and erectile dysfunction in cavernous nerve-injured rats., PMID:24636283

Analysis of candidate genes for lineage-specific expression changes in humans and primates., PMID:24911366

Cardiac CD47 drives left ventricular heart failure through Ca2+-CaMKII-regulated induction of HDAC3., PMID:24922625

Oxidative DNA damage induces the ATM-mediated transcriptional suppression of the Wnt inhibitor WIF-1 in systemic sclerosis and fibrosis., PMID:25185156

Histone deacetylase 3 (HDAC3) plays an important role in retinal ganglion cell death after acute optic nerve injury., PMID:25261965

Interferon regulatory factor 1 and histone H4 acetylation in systemic lupus erythematosus., PMID:25611806

GATA1 induces epithelial-mesenchymal transition in breast cancer cells through PAK5 oncogenic signaling., PMID:25726523

MicroRNA-26a/-26b-COX-2-MIP-2 Loop Regulates Allergic Inflammation and Allergic Inflammation-promoted Enhanced Tumorigenic and Metastatic Potential of Cancer Cells., PMID:25907560

Induction of c-Cbl contributes to anti-cancer effects of HDAC inhibitor in lung cancer., PMID:25980579

Histone Deacetylase 3 and 4 Complex Stimulates the Transcriptional Activity of the Mineralocorticoid Receptor., PMID:26305553

JHDM1D and HDAC1-3 mRNA expression levels in peripheral blood mononuclear cells of patients with systemic lupus erythematosus., PMID:26347123

HDAC3 mediates smoking-induced pancreatic cancer., PMID:26745602

Epigenetic Regulation of the Blimp-1 Gene (Prdm1) in B Cells Involves Bach2 and Histone Deacetylase 3., PMID:26786103

Evaluation of the Therapeutic Potential of the Novel Isotype Specific HDAC Inhibitor 4SC-202 in Urothelial Carcinoma Cell Lines., PMID:27250763

Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes., PMID:27507813

IRF5 is elevated in childhood-onset SLE and regulated by histone acetyltransferase and histone deacetylase inhibitors., PMID:28525378

Deacetylase activity of histone deacetylase 3 is required for productive VDJ recombination and B-cell development., PMID:28739911

HDAC inhibitors enhance the immunotherapy response of melanoma cells., PMID:29137331

Proteomic analysis identifies NPTX1 and HIP1R as potential targets of histone deacetylase-3-mediated neurodegeneration., PMID:29486577

PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation., PMID:29555935

MicroRNA-193b-3p regulates chondrogenesis and chondrocyte metabolism by targeting HDAC3., PMID:29774080

Upregulation of PD‑L1 expression by resveratrol and piceatannol in breast and colorectal cancer cells occurs via HDAC3/p300‑mediated NF‑κB signaling., PMID:30066852

Improved early development of porcine cloned embryos by treatment with quisinostat, a potent histone deacetylase inhibitor., PMID:30587665

EGFR-c-Src-Mediated HDAC3 Phosphorylation Exacerbates Invasion of Breast Cancer Cells., PMID:31430896

FcεRI-HDAC3-MCP1 Signaling Axis Promotes Passive Anaphylaxis Mediated by Cellular Interactions., PMID:31597362

(Curcumin+sildenafil) enhances the efficacy of 5FU and anti-PD1 therapies in vivo., PMID:31985048

Checkpoint regulator B7x is epigenetically regulated by HDAC3 and mediates resistance to HDAC inhibitors by reprogramming the tumor immune environment in colorectal cancer., PMID:32934224

RGFP966, a selective HDAC3 inhibitor, ameliorates allergic and inflammatory responses in an OVA-induced allergic rhinitis mouse model., PMID:33529911

Deacetylase Plus Bromodomain Inhibition Downregulates ERCC2 and Suppresses the Growth of Metastatic Colon Cancer Cells., PMID:33809839

Microbial metabolite butyrate promotes induction of IL-10+IgM+ plasma cells., PMID:35333906

Antibodies of the immunoglobulin a isotype to novel antigens in early axial spondyloarthritis., PMID:36844956

Vitamin D3 mitigates autoimmune inflammation caused by activation of myeloid dendritic cells in SLE., PMID:37702410

Molecular characterization of the B lymphocyte-induced maturation protein-1 (blimp1) gene of common carp (Cyprinus carpio) and its transcription repression involves recruitment of histone deacetylase HDAC3., PMID:37944681

PD-L1 Expression in Neoplastic and Immune Cells of Thymic Epithelial Tumors: Correlations with Disease Characteristics and HDAC Expression., PMID:38672128

The Circadian Clock Component RORA Increases Immunosurveillance in Melanoma by Inhibiting PD-L1 Expression., PMID:38718296

Proteomic identification of a Toxoplasma gondii sporozoite-specific antigen using HDAC3 inhibitor-treated tachyzoites as surrogate., PMID:39802703

Anaerobic metabolism promotes breast cancer survival via Histone-3 Lysine-18 lactylation mediating PPARD axis., PMID:39922804

Acetylation profiling by Iseq-Kac reveals insights into HSC aging and lineage decision., PMID:40419771

Macrophage activation determines muscle wasting in pancreatic cancer., PMID:40442475

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