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Anti-Human HLA-B*27 Antibody (H4H2)

Catalog #:   FHM00210 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1, kappa
Applications: FCM
Accession: P01889
Overview

Catalog No.

FHM00210

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

HLA-B, HLA class I histocompatibility antigen, B alpha chain, Human leukocyte antigen B, HLAB, HLA-B*27

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01889

Applications

FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

H4H2

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # FHM00210 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human HLA-B antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-B antibody monoclonal antibody (Catalog # FHM00210 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

Effects of secukinumab on skeletal microarchitecture and vertebral fractures in patients with axial spondyloarthritis using HR-pQCT., PMID:40481972

HLA-B27 Testing in Clinical Practice: A Retrospective Analysis of Testing Indications and Rheumatology Referral Patterns., PMID:40451272

Anti-PCNA antibodies in psoriatic arthritis: a case-control study., PMID:40417241

Case Report: Ankylosing Spondylitis Complicated With Systemic Lupus Erythematosus., PMID:40349144

The influence of genetic factors on the clinical manifestations and response to systemic treatment of plaque psoriasis., PMID:40095058

Knowledge, Awareness, and Attitudes Regarding Axial Spondylarthritis Among Nonrheumatology Physicians in the United States., PMID:40041999

The effect of ixekizumab treatment on MRI sacroiliac joint structural lesions in patients with radiographic axial spondyloarthritis: post-hoc analysis of a 52-week, randomised, placebo-controlled trial with an active reference arm., PMID:39983751

Predictors of Secukinumab Treatment Response and Continuation in Axial Spondyloarthritis: Results From the EuroSpA Research Collaboration Network., PMID:39892891

Five Cases of Non-radiographic Spondyloarthritis (nr-SpA) of Patients from a Tertiary Care Center in the Philippines: A Case Series., PMID:39758302

Progressive pseudorheumatoid dysplasia involving a novel CCN6 mutation: a case report., PMID:39539552

HLA-DRB1*01 predicts treatment outcome in juvenile idiopathic arthritis: A retrospective-prospective cohort study., PMID:39484788

Reactive Arthritis or HIV-Associated Arthropathy: Is It Important to Differentiate Them?, PMID:39429408

Converting an HLA-B27 flow assay from the BD FACSCanto to the BD FACSLyric., PMID:39287115

Anti-recoverin Antibody-Associated Post-acute COVID Vaccination Syndrome After BNT162b2 in HLA-B27-Positive Spondylarthritis: A Case Report., PMID:39280509

The Structural Basis for Recognition of Human Leukocyte Antigen Class I Molecules by the Pan-HLA Antibody W6/32., PMID:39093013

Four-year real-world experience of secukinumab in a large Italian cohort of axial spondyloarthritis., PMID:39076975

Conformational Alterations of the Cell Surface of Monomeric and Dimeric β2m-Free HLA-I (Proto-HLA) May Enable Novel Immune Functions in Health and Disease., PMID:39057057

Effectiveness of secukinumab in radiographic and non-radiographic axial spondyloarthritis: a European routine-care observational study., PMID:39053949

An Uncommon Association: HLA-B27 Positivity in Conjunction With Pernicious Anemia - A Case Report and Literature Review., PMID:39036579

Course of Uveitis in Patients with Ankylosing Spondylitis during the Interleukin17 Inhibitors Therapy., PMID:38861150

Checkpoint inhibitor immunotherapy induced inflammatory arthritis secondary to Nivolumab and Ipilimumab: a pediatric first., PMID:38685034

The Importance of Pseudo Thrombocytopenia Due to Platelet Cold Agglutination before Surgery, What Should We Do? A Case Report., PMID:38680709

Anti-tumor necrosis factor (aTNF) weaning strategy in juvenile idiopathic arthritis (JIA): does duration matter?, PMID:38443603

Anti-HLA-B7/HLA-B44 strong cross immunoreactivity observed in flow cytometry HLA-B27 immunotyping., PMID:38379087

The enigma of sclera-specific autoimmunity in scleritis., PMID:38368769

Targeted therapies for uveitis in spondyloarthritis: A narrative review., PMID:38309516

Seventy-five years of service: an overview of the College of American Pathologists' proficiency testing program in histocompatibility and identity testing., PMID:38169613

Serum biomarkers and their relationship to axial spondyloarthritis associated with inflammatory bowel diseases., PMID:38168574

Juvenile Idiopathic Arthritis., PMID:38163829

Chlamydia trachomatis and the HLA involvement in the development of infection and disease: a narrative review., PMID:37970191

Cell Surface B2m-Free Human Leukocyte Antigen (HLA) Monomers and Dimers: Are They Neo-HLA Class and Proto-HLA?, PMID:37627243

Adalimumab rapidly controls both anterior and posterior inflammation in patients with ocular Behçet syndrome and non-infectious uveitis refractory to conventional therapy: a prospective, 6-month follow-up investigation., PMID:37555889

Choosing Wisely: The Canadian Rheumatology Association Pediatric Committee's List of Items Physicians and Patients Should Question., PMID:37527858

Human leukocyte antigen - B27 and antibodies to Klebsiella pneumoniae in Ankylosing Spondylitis: associations and clinical outcome., PMID:37440183

The use of a tumor necrosis factor inhibitor-adalimumab-in the management of arthritis in an adolescent with HIV: A case report., PMID:37431718

A Review of Laboratory Practices Using the HLA-B27 Survey by the College of American Pathologists: How Important Is Allele-Level Typing?, PMID:37134231

Genetic and Molecular Distinctions Between Axial Psoriatic Arthritis and Radiographic Axial Spondyloarthritis: Post Hoc Analyses from Four Phase 3 Clinical Trials., PMID:36995469

Outcome of Uveitis in Juvenile Idiopathic Arthritis and Spondyloarthritis Patients - A 5-Year Follow-Up Study., PMID:36842134

Peptide-based vaccine targeting IL17A attenuates experimental spondyloarthritis in HLA-B27 transgenic rats., PMID:36737108

Association of HLA-B27 antigen with clinical and laboratory parameters in patients with juvenile idiopathic arthritis., PMID:36692982

Uveitis in psoriatic arthritis: study of 406 patients in a single university center and literature review., PMID:36635002

Recent updates in enthesitis-related arthritis., PMID:36629936

Primary antibody deficiency-associated arthritis shares features with spondyloarthritis and enteropathic arthritis., PMID:36583733

Development of Inflammatory Bowel Disease in Children With Juvenile Idiopathic Arthritis Treated With Biologics., PMID:36399775

Axial spondyloarthritis patients have altered mucosal IgA response to oral and fecal microbiota., PMID:36248884

Practical Significance of Biomarkers in Axial Spondyloarthritis: Updates on Diagnosis, Disease Activity, and Prognosis., PMID:36232862

The microbiome in HLA-B27-associated disease: implications for acute anterior uveitis and recommendations for future studies., PMID:36058784

Seropositive Neuromyelitis Optica in a Case of Undiagnosed Ankylosing Spondylitis: A Neuro-Rheumatological Conundrum., PMID:35864917

Adalimumab or etanercept as first line biologic therapy in enthesitis related arthritis (ERA) - a drug-survival single centre study spanning 10 years., PMID:35660170

Clinical and laboratory features of patients with undifferentiated spondyloarthritis and ankylosing spondylitis., PMID:35319631

Datasheet
$ 225
Product specifications
50 μg 225 100 μg 372

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Anti-Human HLA-B*27 Antibody (H4H2) [FHM00210]
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