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Anti-DNA/RNA G-quadruplex Structures Antibody (BG4)

Catalog #:   RGK60301 Specific References (67) DATASHEET
Isotype: scFv-FLAG-His
Applications: ELISA, EMSA, IF, ChIP-seq, WB
Expression system: Mammalian Cells
Overview

Catalog No.

RGK60301

Expression system

Mammalian Cells

Species reactivity

General

Isotype

scFv-FLAG-His

Clonality

Monoclonal

Applications

ELISA, EMSA, IF, ChIP-seq, WB

Target

DNA/RNA G-quadruplex, DNA G-quadruplex structures,DNA G4,DNA G-quadruplex structures,DNA G-quadruplex

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

BG4

Note

For research use only.

Data Image
  • SDS-PAGE
    SDS PAGE for DNA G-quadruplex Structures Antibody
  • Immunocytochemistry
    Compound 4d colocalized with G4 DNA and downregulated c-MYC expression in HepG2 cells. (A) Representative images of immunocytochemistry with BG4 antibody. Scale bar = 10 μm.
    Data sourced from citation (PMID: 36640595)
  • Immunofluorescence
    Fig. 7 Preliminary validation of m6 A and G-quadruplex co-localization. A Distribution of co-localized sites at the cellular level pre- and post-infection visualized by confocal immunofluorescence microscopy.
    Data sourced from citation (PMID: 41689056)
References

Comprehensive analysis and validation of m6A-G4 colocalization and its regulatory role in Marek's disease virus infection. [RGK60301]

Design, synthesis and biological evaluation of novel 9-N-substituted-13-alkylberberine derivatives from Chinese. [RGK60301]

G-quadruplex targeting chemical nucleases as a nonperturbative tool for analysis of cellular G-quadruplex DNA., PMID:34189433

Light-induced in situ chemical activation of a fluorescent probe for monitoring intracellular G-quadruplex structures., PMID:34477654

A Bayesian model based computational analysis of the relationship between bisulfite accessible single-stranded DNA in chromatin and somatic hypermutation of immunoglobulin genes., PMID:34491985

DNA Binding Mode Analysis of a Core-Extended Naphthalene Diimide as a Conformation-Sensitive Fluorescent Probe of G-Quadruplex Structures., PMID:34638964

Cockayne Syndrome B Protein Selectively Resolves and Interact with Intermolecular DNA G-Quadruplex Structures., PMID:34855372

An organic molecular compound for in situ identification of mitochondrial G-quadruplexes in live cells., PMID:34940779

Combining Excellent Selectivity with Broad Target Scope: Biosensing with Arrayed Deep Cavitand Hosts., PMID:35302733

Stabilization of a DNA aptamer by ligand binding., PMID:35550917

Modulation of Aptamer-Ligand-Binding by Complementary Oligonucleotides: A G-Quadruplex Anti-Ochratoxin A Aptamer Case Study., PMID:35563267

Sandwich capture ultrasensitive sensor based on biohybrid interface for the detection of Cronobacter sakazakii., PMID:35616722

Native RNA G quadruplex immunoprecipitation (rG4IP) from mammalian cells., PMID:35620077

Spontaneous DNA Synapsis by Forming Noncanonical Intermolecular Structures., PMID:35632001

Epigenomic Features and Potential Functions of K+ and Na+ Favorable DNA G-Quadruplexes in Rice., PMID:35955535

Expanding the Toolbox of Target Directed Bio-Orthogonal Synthesis: In Situ Direct Macrocyclization by DNA Templates., PMID:36437509

DNA G-Quadruplex Recognition In Vitro and in Live Cells by a Structure-Specific Nanobody., PMID:36488193

Non-B-form DNA tends to form in centromeric regions and has undergone changes in polyploid oat subgenomes., PMID:36574697

Mammalian telomeric RNA (TERRA) can be translated to produce valine-arginine and glycine-leucine dipeptide repeat proteins., PMID:36812212

HNRNPU facilitates antibody class-switch recombination through C-NHEJ promotion and R-loop suppression., PMID:36943867

Selection of G-rich ssDNA aptamers for the detection of enterotoxins of the cholera toxin family., PMID:36963555

DECODING COMPLEXITY IN BIOMOLECULAR RECOGNITION OF DNA I-MOTIFS., PMID:37131644

Single-molecule analysis of the improved variants of the G-quadruplex recognition protein G4P., PMID:37214990

Single-Molecule Analysis of the Improved Variants of the G-Quadruplex Recognition Protein G4P., PMID:37373425

Phase Separation Modulates the Formation and Stabilities of DNA Guanine Quadruplex., PMID:37388701

G4access identifies G-quadruplexes and their associations with open chromatin and imprinting control regions., PMID:37400615

G-quadruplex DNA contributes to RNA polymerase II-mediated 3D chromatin architecture., PMID:37427784

Post- and Pre-Radiolabeling Assays for anti Thymidine Cyclobutane Dimers as Intrinsic Photoprobes of Various Types of G-Quadruplexes, Reverse Hoogsteen Hairpins, and Other Non-B DNA Structures., PMID:37459251

Genome-wide mapping of i-motifs reveals their association with transcription regulation in live human cells., PMID:37528048

Unique repetitive nucleic acid structures mirror switch regions in the human IgH locus., PMID:37678746

Evaluation of potential role of R-loop and G-quadruplex DNA in the fragility of c-MYC during chromosomal translocation associated with Burkitt's lymphoma., PMID:37926284

Decoding complexity in biomolecular recognition of DNA i-motifs with microarrays., PMID:37962331

BG4 antibody can recognize telomeric G-quadruplexes harboring destabilizing base modifications and lesions., PMID:38153143

G-quadruplex landscape and its regulation revealed by a new antibody capture method., PMID:38484151

Immunofluorescence microscopy of G-quadruplexes and R-loops., PMID:38521582

Observing G4 formation and its resolution by Pif1 in real time by manipulation under magnetic tweezers., PMID:38521583

Production of the anti-G-quadruplex antibody BG4 for efficient genome-wide analyses: From plasmid quality control to antibody validation., PMID:38521585

A beginner's handbook to identify and characterize i-motif DNA., PMID:38521590

A dual amplified gold nanoparticle-based biosensor for ultrasensitive and selective detection of fibrin., PMID:38684508

Development of a single-domain antibody to target a G-quadruplex located on the hepatitis B virus covalently closed circular DNA genome., PMID:38804172

Evolution of a bispecific G-quadruplex-forming circular aptamer to block IL-6/sIL-6R interaction for inflammation inhibition., PMID:39148786

Artificially inserted strong promoter containing multiple G-quadruplexes induces long-range chromatin modification., PMID:39158543

Unveiling a Potent Small Molecule Disruptor for RNA G-Quadruplexes Tougher Than DNA G-Quadruplex Disruption., PMID:39225324

Improved simultaneous mapping of epigenetic features and 3D chromatin structure via ViCAR., PMID:39227991

A Rationally Designed Azobenzene Photoswitch for DNA G-Quadruplex Regulation in Live Cells., PMID:39268751

G-quadruplexes in an SVA retrotransposon cause aberrant TAF1 gene expression in X-linked dystonia parkinsonism., PMID:39287133

Untargeted CUT&Tag and BG4 CUT&Tag are both enriched at G-quadruplexes and accessible chromatin., PMID:39386625

Ultrasensitive and high selectivity detection of fibrin using Y-shaped DNA-homing peptide doped probe on localized surface plasmon resonance platform., PMID:39788687

The iMab antibody selectively binds to intramolecular and intermolecular i-motif structures., PMID:39817511

Evaluating the Performance of Peak Calling Algorithms Available for Intracellular G-Quadruplex Sequencing., PMID:39941033

Analysis of quadruplex propensity of aptamer sequences., PMID:40377215

Structural basis of G-quadruplex recognition by a camelid antibody fragment., PMID:40433978

Chen, J., Duan, Y., Yang, K., Wang, J., Yan, J., Gu, C., Wang, S., Zhu, Z., Liu, E.H., and Xu, J. (2023). Design, synthesis and biological evaluation of novel 9-N-substituted-13-alkylberberine derivatives from Chinese medicine as anti-hepatocellular carcinoma agents. Bioorg Med Chem 79, 117156. PMID: 36640595

GNG Motifs Can Replace a GGG Stretch during G-Quadruplex Formation in a Context Dependent Manner. PMID: 27414642

Elevated levels of G-quadruplex formation in human stomach and liver cancer tissues. PMID: 25033211

An organic molecular compound for in situ identification of mitochondrial G-quadruplexes in live cells. PMID: 34940779

Small Molecule Driven Stabilization of Promoter G-Quadruplexes and Transcriptional Regulation of c-MYC. PMID: 29956928

Telomere and G-Quadruplex Colocalization Analysis by Immunofluorescence Fluorescence In Situ Hybridization (IF-FISH). PMID: 31127589

Activation-induced cytidine deaminase localizes to G-quadruplex motifs at mutation hotspots in lymphoma. PMID: 33094287

Light-induced in situ chemical activation of a fluorescent probe for monitoring intracellular G-quadruplex structures. PMID: 34477654

Subcellular Duplex DNA and G-Quadruplex Interaction Profiling of a Hexagonal PtII Metallacycle. PMID: 31002438

G4 Sensing Pyridyl-Thiazole Polyamide Represses c-KIT Expression in Leukemia Cells. PMID: 33851760

Cockayne Syndrome B Protein Selectively Resolves and Interact with Intermolecular DNA G-Quadruplex Structures. PMID: 34855372

BG-flow, a new flow cytometry tool for G-quadruplex quantification in fixed cells. PMID: 33706790

[Preparation and identification of DNA G-quadruplex antibody]. PMID: 26146071

Custom DNA Microarrays Reveal Diverse Binding Preferences of Proteins and Small Molecules to Thousands of G-Quadruplexes. PMID: 32216326

Characterization of G-quadruplex antibody reveals differential specificity for G4 DNA forms. PMID: 33084858

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

Contact Information

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For research use only. Not for human or drug use.

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Anti-DNA/RNA G-quadruplex Structures Antibody (BG4) [RGK60301]
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