Anti-Human CD47/MER6 Antibody (B6H12)

Catalog #: FHG17620 Specific References (0) DATASHEET

Host species: Mouse

Isotype: IgG1, kappa

Applications: FCM

Accession: Q08722

Overview

Catalog No.

FHG17620

Species reactivity

Human

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q08722

Applications

FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

B6H12

Data Image

Bioactivity

Detects CD47/MER6 in indirect ELISAs.
Flow-cytometry

Flow-cytometry using anti-human CD47 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # FHG17620 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD47 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # FHG17620 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD47 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # FHG17620 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD47 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 antibody monoclonal antibody (Catalog # FHG17620 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Document Download

Anti-Human CD47/MER6 Antibody (B6H12).pdf

$ 198

Product specifications

50 μg 198 100 μg 328

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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