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Anti-Human CD3E Antibody (UCHT-1) (FHC27720)

Host species:Rabbit
Isotype:IgG, kappa
Applications:FCM
Accession:P07766
Overview

Catalog No.

FHC27720

Species reactivity

Human

Host species

Rabbit

Isotype

IgG, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain,UCHT1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P07766

Applications

FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

UCHT-1

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human CD3E antibody.CD3E Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # FHC27720 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # PTB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human CD3E antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # FHC27720 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # PTB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human CD3E antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E antibody monoclonal antibody (Catalog # FHC27720 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # PTB96441) and cells analysed on a NovoCyte Flow Cytometer.
References
Datasheet
$ 120
Product specifications
50 μg 120 100 μg 198

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human CD3E Antibody (UCHT-1) [FHC27720]
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