Anti-Human EGFR/ERBB1/HER1 Antibody (E7.6.3) (FHB86920)

specific references (0) DATASHEET

Host species：Human

Isotype：IgG2, kappa

Applications：FCM

Accession：P00533

Overview

Catalog No.

FHB86920

Species reactivity

Human

Host species

Human

Isotype

IgG2, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P00533

Applications

FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

E7.6.3

Data Image

Flow-cytometry

Flow-cytometry using anti-human EGFR antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # FHB86920 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human EGFR antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # FHB86920 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human EGFR antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EGFR antibody monoclonal antibody (Catalog # FHB86920 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Document Download

Anti-Human EGFR/ERBB1/HER1 Antibody (E7.6.3).pdf

$ 225

Product specifications

50 μg 225 100 μg 372

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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