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Anti-Human HLA-A,B,C Antibody (W6/32) (FHM00110)

Host species:Mouse
Isotype:IgG2a, kappa
Applications:ELISA, FCM, ICC, IHC-Fr, IP, WB
Overview

Catalog No.

FHM00110

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Concentration

6 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

ELISA, FCM, ICC, IHC-Fr, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

W6/32

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human HLA-A,B,C Monomorphic antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-A,B,C Monomorphic antibody monoclonal antibody (Catalog # FHM00110 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human HLA-A,B,C antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human HLA-A,B,C antibody monoclonal antibody (Catalog # FHM00110 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

Xiang, H., Zhang, L., Bu, F., Guan, X., Chen, L., Zhang, H., Zhao, Y., Chen, H., Zhang, W., and Li, Y. (2022). A novel proteogenomic integration strategy expands the breadth of neo-epitope sources. Cancers 14, 3016. PMID: 35740681

Chen, L., Zhang, Y., Yang, Y., Yang, Y., Li, H., Dong, X., Wang, H., Xie, Z., and Zhao, Q. (2021). An integrated approach for discovering noncanonical MHC-I peptides encoded by small open reading frames. Journal of the American Society for Mass Spectrometry 32, 2346-2357. PMID: 34260243

Datasheet
$ 225
Product specifications
50 μg 225 100 μg 372

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human HLA-A,B,C Antibody (W6/32) [FHM00110]
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