Anti-Human NOTCH2/3 Antibody (SAA0143) (FHG01810)

specific references (0) DATASHEET

Host species：Human

Isotype：IgG2, kappa

Applications：FCM

Accession：Q04721, Q9UM47

Overview

Catalog No.

FHG01810

Species reactivity

Human

Host species

Human

Isotype

IgG2, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q04721, Q9UM47

Applications

FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0143

Data Image

Flow-cytometry

Flow-cytometry using anti-human NOTCH2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # FHG01810 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human NOTCH3 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH3 antibody monoclonal antibody (Catalog # FHG01810 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human NOTCH2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 antibody monoclonal antibody (Catalog # FHG01810 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human NOTCH2 & NOTCH3 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human NOTCH2 & NOTCH3 antibody monoclonal antibody (Catalog # FHG01810 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Document Download

Anti-Human NOTCH2/3 Antibody (SAA0143).pdf

$ 225

Product specifications

50 μg 225 100 μg 372

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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