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Anti-Human VIM/Vimentin Antibody (VB6) (FHC35310)

Host species:Human
Isotype:IgG1, kappa
Applications:ELISA, FCM, WB
Accession:P08670
Overview

Catalog No.

FHC35310

Species reactivity

Human

Host species

Human

Isotype

IgG1, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

Vimentin, VIM, RHC35301

Concentration

1.86 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08670

Applications

ELISA, FCM, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

VB6

Data Image
  • Bioactivity
    Detects VIM/Vimentin in indirect ELISAs.
  • Flow-cytometry
    Flow-cytometry using anti-human VIM antibody.VIM/Vimentin Transfected HI5 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # FHC35310 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human VIM antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # FHC35310 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • SEC-HPLC
    The purity of this product is >95% as determined by SEC-HPLC.
References
Datasheet
$ 225
Product specifications
50 μg 225 100 μg 372

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human VIM/Vimentin Antibody (VB6) [FHC35310]
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