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MBdSMC whole cell lysate

Catalog #:   CLM00019 Specific References (50) DATASHEET
Applications: WB
Overview

Catalog No.

CLM00019

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Data Image
References

Profiling Ligand-Induced Changes in Nuclear Localization Using Proximity Labeling-Coupled Chemoproteomics., PMID:40515985

Analysis of isobaric quantitative proteomic data using TMT-Integrator and FragPipe computational platform., PMID:40501722

Ageing Signatures and Disturbed Muscle Regeneration in Muscle Proteome of Inclusion Body Myositis., PMID:40485189

Potent Cyclic Peptide Inhibitors Disrupt the FANCM-RMI Interaction., PMID:40479515

Proteomics Analysis and Sequential Events During the in-vivo Acquisition of Drug Resistance in Clinical Isolates of Mycobacterium tuberculosis., PMID:40417764

A general and accessible approach to enrichment and characterisation of natural anti-Neu5Gc antibodies from human samples., PMID:40416449

Multiplex Immunoassay for Biomarker Profiling of Whole Blood Cell Lysates and Supernatants and Pathogen Response in Neat Whole Blood Cultures., PMID:40407473

S-palmitoylation coordinates the trafficking of ATG9A to mediate autophagy initiation., PMID:40394978

Biocatalytic Thioketal Cleavage Enabled by Enzymatic Bromide Recycling by Vanadium-Dependent Haloperoxidases., PMID:40393052

Discovery of new protein partners of anterior gradient 2 (AGR2) from cell-cell adhesion pathway using genetically-encoded photo-crosslinker DiZPK., PMID:40367911

TempO-seq and RNA-seq gene expression levels are highly correlated for most genes: A comparison using 39 human cell lines., PMID:40344165

Human platelet lysate produced from leukoreduction filter contents enables sufficient MSC growth., PMID:40270024

Current Landscape of Therapeutic Cancer Vaccines., PMID:40266513

Biocatalytic production of 3-hydroxypropionic acid precursors using a regioselective Baeyer-Villiger monooxygenase., PMID:40263398

Emergence and characterization of IncFII/IncR plasmids with multiple 5,692 bp- bla KPC-2-bearing tandem repeats in ceftazidime/avibactam non-susceptible Klebsiella pneumoniae strains., PMID:40248432

One-Pot Quantitation of Mycobacterial N-terminal Protein Acetylation Peptidoforms and Proteome., PMID:40236102

An in vivo "turning model" reveals new RanBP9 interactions in lung macrophages., PMID:40223093

Oligosaccharide production using β-galactosidase from Lactobacillus bulgaricus and Kluyveromyces lactis in sweetened reconstituted nonfat dry milk., PMID:40222678

Efficient degradation of alternariol in food by a novel isolate, Bacillus pacificus ANSB901., PMID:40210405

Extracellular Vesicles Released From Skeletal Muscle Post-Chronic Contractile Activity Increase Mitochondrial Biogenesis in Recipient Myoblasts., PMID:40205946

A method for the detection and enrichment of endogenous cereblon substrates., PMID:40196695

Quantification of Total Free Radicals in Drosophila Using a Fluorescence-Based Biochemical Assay., PMID:40084083

Comparison of immunogenicity of 17 Burkholderia mallei antigens and whole cell lysate using indirect ELISA., PMID:40044168

Differential anaerobic oxidation of benzoate in Geotalea daltonii FRC-32., PMID:40042335

Detection of human unannotated microproteins by mass spectrometry-based proteomics: a community assessment., PMID:40027765

A novel, rapidly progressive ataxia due to a spontaneous Myo5a mutation in mice impairs transport proteins and alters mitochondria., PMID:40022605

Comprehensive Protein Inference Analysis with PyProteinInference Elucidates Biological Understanding of Tandem Mass Spectrometry Data., PMID:40019342

A bacterial RING ubiquitin ligase triggering stepwise degradation of BRISC via TOLLIP-mediated selective autophagy manipulates host inflammatory response., PMID:40013521

The Cortisol Effect on the NO/cGMP Pathway., PMID:40003888

Evaluation of mucosal adjuvants to chitosan-nanoparticle-based oral subunit vaccine for controlling salmonellosis in broilers., PMID:39981235

GTPBP2 in-frame deletion in canine model with non-syndromic progressive retinal atrophy., PMID:39971978

Whole-Component Antigen Nanovaccines Combined With aTIGIT for Enhanced Innate and Adaptive Anti-tumor Immunity., PMID:39967373

Role of endosomal RANKL-LGR4 signaling during osteoclast differentiation., PMID:39954074

Killed whole-cell Staphylococcus aureus formulation in Montanide ISA266 and Alum adjuvants: different vaccine formulations varied in the vaccine's potency and efficacy., PMID:39918699

Theranostic Near-Infrared Monoamine Oxidase Inhibitor (NMI) Protein Binding Interactions with MAOA and Albumin., PMID:39904854

Incremental Modification in the Existing Approaches for Affinity Chromatographic Enrichment of Phosphoproteins Improves Their Profile in Liquid Chromatography-Tandem Mass Spectrometry Analysis., PMID:39902464

Proteome-Wide Profiling of Olaparib Interactors Using a Biotinylated Photoaffinity Probe., PMID:39898787

Novel biallelic ADCY10 variants cause asthenozoospermia with excessive residual cytoplasm and hydronephrosis in humans., PMID:39891992

Durable suppression of seizures in a preclinical model of KCNT1 genetic epilepsy with divalent small interfering RNA., PMID:39871703

Microbial lysates as low-cost serum replacements in cellular agriculture media formulation., PMID:39849780

Total cell N-glycosylation is altered during differentiation of induced pluripotent stem cells to neural stem cells and is disturbed by trisomy 21., PMID:39845703

A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases., PMID:39833196

Validation of an in house iELISA for serodiagnosis of caprine brucellosis and evaluation of the performance of a B. neotomae lysate for the detection of anti-smooth Brucella specific antibodies in ruminants., PMID:39826311

Virus Association with Bacteria and Bacterial Cell Components Enhance Virus Infectivity., PMID:39789292

Current trends in the design and fabrication of PRP-based scaffolds for tissue engineering and regenerative medicine., PMID:39787704

Comparison of ELISA Versus FAMA Titers in Children After Chemotherapy and Hematopoietic Stem Cell Transplantation Who Received the Live Attenuated MAV/06 Strain Varicella Vaccine., PMID:39772033

Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis., PMID:39702171

Analyses of biosynthesis mutants reveal that the fifth and sixth sugars of the Porphyromonas gingivalis O-glycan are L-fucose and N-acetylgalactosamine respectively., PMID:39701196

Extraction of Total Protein from Cardiomyocytes and Western Blotting Analysis., PMID:39699817

Blocking autophagosome closure manifests the roles of mammalian Atg8-family proteins in phagophore formation and expansion during nutrient starvation., PMID:39694607

Datasheet

Document Download

MBdSMC whole cell lysate.pdf

 

$ 250
Product specifications
100 μg 250

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For research use only. Not for human or drug use.

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MBdSMC whole cell lysate [CLM00019]
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