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LLC whole cell lysate

Catalog #:   CLM00016 Specific References (20) DATASHEET
Applications: WB
Overview

Catalog No.

CLM00016

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Data Image
References

A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases., PMID:39833196

Colorectal carcinoma cell targeting aromatherapy with Teucrium ramosissimum essential oil to sensitize TRAIL/Apo2L-induced HCT-116 cell death., PMID:38850792

One-pot method for preparing DNA, RNA, and protein for multiomics analysis., PMID:38485785

Fluorescence Polarization-Based Measurement of Protein-Ligand Interaction in Fungal Cell Lysates., PMID:33484500

Engineered Nanoparticles for Cancer Vaccination and Immunotherapy., PMID:33017150

Development of a TCR beta repertoire assay for profiling liquid biopsies from NSCLC donors., PMID:35582444

Silencing IDO2 in dendritic cells: A novel strategy to strengthen cancer immunotherapy in a murine lung cancer model., PMID:32468023

Irradiation increases the immunogenicity of lung cancer cells and irradiation-based tumor cell vaccine elicits tumor-specific T cell responses in vivo., PMID:31190880

Immunoglobulin G from single plasma donor in immune globulin intravenous causes false positive pyrogen test., PMID:31023510

The VICM biomarker is released from activated macrophages and inhibited by anti-GM-CSFRα-mAb treatment in rheumatoid arthritis patients., PMID:30418117

Post-operative unadjuvanted therapeutic xenovaccination with chicken whole embryo vaccine suppresses distant micrometastases and prolongs survival in a murine Lewis lung carcinoma model., PMID:29552144

Bacterial ghosts as adjuvants in syngeneic tumour cell lysate-based anticancer vaccination in a murine lung carcinoma model., PMID:27878261

Evaluation of microparticulate ovarian cancer vaccine via transdermal route of delivery., PMID:27238440

Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge study., PMID:26988085

Competitive, immunometric assay for fusion protein quantification: protein production prioritization., PMID:24121012

Investigating evolutionary perspective of carcinogenesis with single-cell transcriptome analysis., PMID:23706768

Efficient responses in a murine renal tumor model by electroloading dendritic cells with whole-tumor lysate., PMID:16224271

Detection of cystic fibrosis mutations by peptide mass signature genotyping., PMID:12881448

Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence., PMID:8157605

Transcriptional regulation of a plasminogen activator gene by cyclic AMP in a homologous cell-free system. Involvement of cyclic AMP-dependent protein kinase in transcriptional control., PMID:2828371

Datasheet

Document Download

LLC whole cell lysate.pdf

 

$ 250
Product specifications
100 μg 250

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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LLC whole cell lysate [CLM00016]
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