Catalog No.
CLM00016
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
A customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases., PMID:39833196
Colorectal carcinoma cell targeting aromatherapy with Teucrium ramosissimum essential oil to sensitize TRAIL/Apo2L-induced HCT-116 cell death., PMID:38850792
One-pot method for preparing DNA, RNA, and protein for multiomics analysis., PMID:38485785
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Silencing IDO2 in dendritic cells: A novel strategy to strengthen cancer immunotherapy in a murine lung cancer model., PMID:32468023
Irradiation increases the immunogenicity of lung cancer cells and irradiation-based tumor cell vaccine elicits tumor-specific T cell responses in vivo., PMID:31190880
Immunoglobulin G from single plasma donor in immune globulin intravenous causes false positive pyrogen test., PMID:31023510
The VICM biomarker is released from activated macrophages and inhibited by anti-GM-CSFRα-mAb treatment in rheumatoid arthritis patients., PMID:30418117
Post-operative unadjuvanted therapeutic xenovaccination with chicken whole embryo vaccine suppresses distant micrometastases and prolongs survival in a murine Lewis lung carcinoma model., PMID:29552144
Bacterial ghosts as adjuvants in syngeneic tumour cell lysate-based anticancer vaccination in a murine lung carcinoma model., PMID:27878261
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Comparative analysis of routes of immunization of a live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous virus challenge study., PMID:26988085
Competitive, immunometric assay for fusion protein quantification: protein production prioritization., PMID:24121012
Investigating evolutionary perspective of carcinogenesis with single-cell transcriptome analysis., PMID:23706768
Efficient responses in a murine renal tumor model by electroloading dendritic cells with whole-tumor lysate., PMID:16224271
Detection of cystic fibrosis mutations by peptide mass signature genotyping., PMID:12881448
Nucleotide sequence of the rhamnose biosynthetic operon of Shigella flexneri 2a and role of lipopolysaccharide in virulence., PMID:8157605
Transcriptional regulation of a plasminogen activator gene by cyclic AMP in a homologous cell-free system. Involvement of cyclic AMP-dependent protein kinase in transcriptional control., PMID:2828371