Catalog No.
CLH00149
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Leveraging a High-Throughput Screening Method to Identify Mechanisms of Individual Susceptibility Differences in a Genetically Diverse Zebrafish Model., PMID:35573279
Human Platelet Lysate for Good Manufacturing Practice-Compliant Cell Production., PMID:34068404
Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction., PMID:29488993
LcrV delivered via type III secretion system of live attenuated Yersinia pseudotuberculosis enhances immunogenicity against pneumonic plague., PMID:25114109
Quantitative determination of erythrocyte folate vitamer distribution by liquid chromatography-tandem mass spectrometry., PMID:16599840
T-cell responses of leprosy patients and healthy contacts toward separated protein antigens of Mycobacterium leprae., PMID:1602193
Human T lymphocytes and monocytes bear the same Leu-3(T4) antigen., PMID:3084644
For research use only. Not for human or drug use.
