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A375 whole cell lysate

Catalog #:   CLH00138 Specific References (3) DATASHEET
Applications: WB
Overview

Catalog No.

CLH00138

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Data Image
References

Proteomics pipeline for phosphoenrichment and its application on a human melanoma cell model., PMID:32928406

A novel interaction between calcium-modulating cyclophilin ligand and Basigin regulates calcium signaling and matrix metalloproteinase activities in human melanoma cells., PMID:23879967

Dendritic cells, pulsed with lysate of allogeneic tumor cells, are capable of stimulating MHC-restricted antigen-specific antitumor T cells., PMID:16720928

Datasheet

Document Download

A375 whole cell lysate.pdf

 

$ 167
Product specifications
100 μg 167

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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A375 whole cell lysate [CLH00138]
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