Catalog No.
CLH00128
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Development of the T-ALLiPSC-based therapeutic cancer vaccines for T-cell acute lymphoblastic leukemia., PMID:36173527
Amorphous TiO2 Nanotubes as a Platform for Highly Selective Phosphopeptide Enrichment., PMID:31460330
3D HPLC-MS with Reversed-Phase Separation Functionality in All Three Dimensions for Large-Scale Bottom-Up Proteomics and Peptide Retention Data Collection., PMID:26849966
Transcription factor ICBP90 regulates the MIF promoter and immune susceptibility locus., PMID:26752645
Effects of Helicobacter suis γ-glutamyl transpeptidase on lymphocytes: modulation by glutamine and glutathione supplementation and outer membrane vesicles as a putative delivery route of the enzyme., PMID:24147103
p85-RhoGDI2, a novel complex, is required for PSGL-1-induced β1 integrin-mediated lymphocyte adhesion to VCAM-1., PMID:24055812
[Novel complexes of gene expression and their role in the appearance and evolution of the genus Homo]., PMID:23795460
Fluorescent coumarin thiols measure biological redox couples., PMID:22264005
Rip1 (receptor-interacting protein kinase 1) mediates necroptosis and contributes to renal ischemia/reperfusion injury., PMID:22237751
iProphet: multi-level integrative analysis of shotgun proteomic data improves peptide and protein identification rates and error estimates., PMID:21876204
[TRPV5 and TRPV6 calcium channels in human T cells]., PMID:19140341
The apoptosis modulator and tumour suppressor protein RBM5 is a phosphoprotein., PMID:16927403
Enrichment and analysis of peptide subsets using fluorous affinity tags and mass spectrometry., PMID:15768030
Phosphotyrosine proteomic study of interferon alpha signaling pathway using a combination of immunoprecipitation and immobilized metal affinity chromatography., PMID:15659558
Increase of nuclear ceramide through caspase-3-dependent regulation of the "sphingomyelin cycle" in Fas-induced apoptosis., PMID:14871831
Two-dimensional Blue native/SDS gel electrophoresis of multi-protein complexes from whole cellular lysates: a proteomics approach., PMID:14665681
Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry., PMID:12522270
CXCR4 heterogeneity in primary cells: possible role of ubiquitination., PMID:12488503
TRAIL-induced apoptosis is independent of the mitochondrial apoptosis mediator DAP3., PMID:12359235
The human immunodeficiency virus (HIV)-1 protease inhibitor saquinavir inhibits proteasome function and causes apoptosis and radiosensitization in non-HIV-associated human cancer cells., PMID:12234989
Electromagnetic field effects: changes in protein phosphorylation in the Jurkat E6.1 cell line., PMID:11347389
Caspase-1 is not involved in CD95/Fas-induced apoptosis in Jurkat T cells., PMID:9925765
CD20 is a molecular target for scFvFc:zeta receptor redirected T cells: implications for cellular immunotherapy of CD20+ malignancy., PMID:9763110
Prolactin is an autocrine growth factor for the Jurkat human T-leukemic cell line., PMID:9357442
Activation of Src-family tyrosine kinases during Fas-induced apoptosis., PMID:8864141
Exogenous human immunodeficiency virus type-1 Tat protein selectively stimulates a phosphatidylinositol-specific phospholipase C nuclear pathway in the Jurkat T cell line., PMID:7589147
Transcriptional regulation of CD6 expression on human T lymphocytes by phorbol ester., PMID:8207228
"Spot transfer", elution and comigration with known proteins allows accurate transferral of protein identifications between distinct two-dimensional electrophoretic systems., PMID:8055881
Response of Jurkat T cells to phorbol ester and bryostatin. Development of sublines with distinct functional responses and changes in protein kinase C activity., PMID:1834742
For research use only. Not for human or drug use.
