U937 whole cell lysate

Catalog No.

CLH00126

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Human ULK1 Variation and Susceptibility to Mycobacterium tuberculosis Infection., PMID:27485354

Arbutin, an intracellular hydroxyl radical scavenger, protects radiation-induced apoptosis in human lymphoma U937 cells., PMID:25187044

Burkholderia cenocepacia lipopolysaccharide, lipid A, and proinflammatory activity., PMID:15044201

Leishmania major induces deactivation of extracellular signal regulated kinases 2 in human U937 macrophage like cells., PMID:14658227

Eliciting cytotoxic T lymphocytes against acute myeloid leukemia-derived antigens: evaluation of dendritic cell-leukemia cell hybrids and other antigen-loading strategies for dendritic cell-based vaccination., PMID:12111118

Attenuation of gamma interferon-induced tyrosine phosphorylation in mononuclear phagocytes infected with Leishmania donovani: selective inhibition of signaling through Janus kinases and Stat1., PMID:7591091

Partial amino acid sequence of a novel protozoan parasite antigen that inhibits non-specific cytotoxic cell activity., PMID:7519359

C5a as a model for chemotactic factor-stimulated tyrosine phosphorylation in the human neutrophil., PMID:8133059

The heparin binding PECAM-1 adhesion molecule is expressed by CD34+ hematopoietic precursor cells with early myeloid and B-lymphoid cell phenotypes., PMID:7693043

Evidence for the involvement of multiple signalling pathways in C5a-induced actin polymerization and nucleation in human monocyte-like cells., PMID:1883486

Induction of tissue factor-like activity of human monoblastic leukemia cell line by tumor necrosis factor-alpha., PMID:2559490

Tumor necrosis factor induces tissue factor-like activity in human leukemia cell line U937 and peripheral blood monocytes., PMID:3134064

Monoclonal antibodies that bind to the My23 human myeloid cell surface molecule: epitope analysis and antigen modulation studies., PMID:2441245

Human T lymphocytes and monocytes bear the same Leu-3(T4) antigen., PMID:3084644

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