HL60 whole cell lysate

Catalog No.

CLH00123

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Gamma irradiation of human leukaemic cells HL-60 and MOLT-4 induces decrease in Mcl-1 and Bid, release of cytochrome c, and activation of caspase-8 and caspase-9., PMID:18470751

Brusatol-induced HL-60 cell differentiation involves NF-kappaB activation., PMID:15019158

A 28-kDa glycoprotein functions as a platelet ligand for P-selectin (CD62P)., PMID:12008955

Nuclear factor-kappaB activation by the photochemotherapeutic agent verteporfin., PMID:10607710

'Loop' domain deletional mutant of Bcl-xL is as effective as p29Bcl-xL in inhibiting radiation-induced cytosolic accumulation of cytochrome c (cyt c), caspase-3 activity, and apoptosis., PMID:10030271

p40(phox) is phosphorylated on threonine 154 and serine 315 during activation of the phagocyte NADPH oxidase. Implication of a protein kinase c-type kinase in the phosphorylation process., PMID:9804763

Role of prolactin in the in vitro development of interleukin-2-driven anti-tumoural lymphokine-activated killer cells., PMID:9014831

The chemopreventive flavonoid apigenin induces G2/M arrest in keratinocytes., PMID:8968050

Pyrrolidine dithiocarbamate, a potent inhibitor of nuclear factor kappa B (NF-kappa B) activation, prevents apoptosis in human promyelocytic leukemia HL-60 cells and thymocytes., PMID:7986199

Differential induction of etoposide-mediated apoptosis in human leukemia HL-60 and K562 cells., PMID:7969039

Partial amino acid sequence of a novel protozoan parasite antigen that inhibits non-specific cytotoxic cell activity., PMID:7519359

Luminol and diazoluminomelanin as indicators of HL-60 cell differentiation., PMID:8407717

Monoclonal antibodies that bind to the My23 human myeloid cell surface molecule: epitope analysis and antigen modulation studies., PMID:2441245

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