Catalog No.
CLH00105
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Glycan node profiling of soluble and membrane glycoproteins in whole cell lysates., PMID:37699507
Cannabidiol-Driven Alterations to Inflammatory Protein Landscape of Lipopolysaccharide-Activated Macrophages In Vitro May Be Mediated by Autophagy and Oxidative Stress., PMID:33998893
Human Cytomegalovirus Interactions with the Basement Membrane Protein Nidogen 1., PMID:33177203
Targeting the Bacterial Cytoskeleton of the Burkholderia cepacia Complex for Antimicrobial Development: A Cautionary Tale., PMID:29848957
AGE-albumin enhances ABCA1 degradation by ubiquitin-proteasome and lysosomal pathways in macrophages., PMID:29097054
Microproteomics with microfluidic-based cell sorting: Application to 1000 and 100 immune cells., PMID:28556466
Alpha 1-antitrypsin does not inhibit human monocyte caspase-1., PMID:25658455
Pleiotropic effects of Blastocystis spp. Subtypes 4 and 7 on ligand-specific toll-like receptor signaling and NF-κB activation in a human monocyte cell line., PMID:24551212
Ehrlichia chaffeensis transcriptome in mammalian and arthropod hosts reveals differential gene expression and post transcriptional regulation., PMID:21915290
A novel strategy to screen Bacillus Calmette-Guérin protein antigen recognized by γδ TCR., PMID:21526117
NCX 4040, a nitric oxide-donating aspirin, exerts anti-inflammatory effects through inhibition of I kappa B-alpha degradation in human monocytes., PMID:20065114
Inhibition of carboxylesterase activity of THP1 monocytes/macrophages and recombinant human carboxylesterase 1 by oxysterols and fatty acids., PMID:19761868
Surface-exposed proteins of Ehrlichia chaffeensis., PMID:17517859
Ni(II) activates the Nrf2 signaling pathway in human monocytic cells., PMID:16806455
Effect of peptide aldehydes with IL-1 beta converting enzyme inhibitory properties on IL-1 alpha and IL-1 beta production in vitro., PMID:8964658
Induction of tissue factor-like activity of human monoblastic leukemia cell line by tumor necrosis factor-alpha., PMID:2559490
For research use only. Not for human or drug use.
