A431 whole cell lysate

Catalog No.

CLH00081

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

A clean, more efficient method for in-solution digestion of protein mixtures without detergent or urea., PMID:17137347

Identification and characterization of differentially active pools of type IIalpha phosphatidylinositol 4-kinase activity in unstimulated A431 cells., PMID:12954081

Vascular endothelial growth factor promotes tumor dissemination by a mechanism distinct from its effect on primary tumor growth., PMID:8631034

In vitro phosphorylation of angiotensin analogs by tyrosyl protein kinases., PMID:6822491

Document Download

A431 whole cell lysate.pdf