Catalog No.
CLH00057
Concentration
100 µg at 2 mg/ml
Applications
WB
Form
Liquid
Storage buffer
1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Discovery of new protein partners of anterior gradient 2 (AGR2) from cell-cell adhesion pathway using genetically-encoded photo-crosslinker DiZPK., PMID:40367911
The Role of Insulin-like Growth Factor Binding Protein (IGFBP)-2 in DNA Repair and Chemoresistance in Breast Cancer Cells., PMID:38893232
The ancestral type of the R-RAS protein has oncogenic potential., PMID:38383288
Chemical synthesis and biochemical properties of cholestane-5α,6β-diol-3-sulfonate: A non-hydrolysable analogue of cholestane-5α,6β-diol-3β-sulfate., PMID:37683773
Similar deamination activities but different phenotypic outcomes induced by APOBEC3 enzymes in breast epithelial cells., PMID:37324648
2-Difluoromethoxy-Substituted Estratriene Sulfamates: Synthesis, Antiproliferative SAR, Antitubulin Activity, and Steroid Sulfatase Inhibition., PMID:36109340
Bioprospecting the microbiome of Red Sea Atlantis II brine pool for peptidases and biosynthetic genes with promising antibacterial activity., PMID:35655185
Leveraging a High-Throughput Screening Method to Identify Mechanisms of Individual Susceptibility Differences in a Genetically Diverse Zebrafish Model., PMID:35573279
Design and synthesis of Aza-boeravinone derivatives as potential novel topoisomerase I inhibitors., PMID:35338969
Putative N-glycoprotein markers of MCF-7/ADR cancer stem cells from N-glycoproteomics characterization of the whole cell lysate., PMID:34074422
A novel method to detect intracellular metabolite alterations in MCF-7 cells by doxorubicin induced cell death., PMID:33389242
Optimization of a WGA-Free Molecular Tagging-Based NGS Protocol for CTCs Mutational Profiling., PMID:32575430
Induction of G2/M Phase Arrest by Diosgenin via Activation of Chk1 Kinase and Cdc25C Regulatory Pathways to Promote Apoptosis in Human Breast Cancer Cells., PMID:31881805
Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool., PMID:30885206
Proline dehydrogenase-entrapped mesoporous magnetic silica nanomaterial for electrochemical biosensing of L-proline in biological fluids., PMID:28756863
β-Catenin Mediates Anti-adipogenic and Anticancer Effects of Arctigenin in Preadipocytes and Breast Cancer Cells., PMID:28279068
Thermodynamic Analysis of the Geldanamycin-Hsp90 Interaction in a Whole Cell Lysate Using a Mass Spectrometry-Based Proteomics Approach., PMID:27530778
Femto-molar detection of cancer marker-protein based on immuno-nanoplasmonics at single-nanoparticle scale., PMID:27009431
Solid-phase extraction and purification of membrane proteins using a UV-modified PMMA microfluidic bioaffinity μSPE device., PMID:24487280
Progesterone receptor-B enhances estrogen responsiveness of breast cancer cells via scaffolding PELP1- and estrogen receptor-containing transcription complexes., PMID:24469035
Taxol-induced growth arrest and apoptosis is associated with the upregulation of the Cdk inhibitor, p21WAF1/CIP1, in human breast cancer cells., PMID:23023313
Pterostilbene induces mitochondrially derived apoptosis in breast cancer cells in vitro., PMID:22572619
Analyzing effects of extra-virgin olive oil polyphenols on breast cancer-associated fatty acid synthase protein expression using reverse-phase protein microarrays., PMID:18813848
[BRCA1 regulates progesterone receptors A and B protein expressions in breast cancer cells in vitro]., PMID:18676251
Identification of estrogen-responsive proteins in MCF-7 human breast cancer cells using label-free quantitative proteomics., PMID:18491314
Exploring the precursor ion exclusion feature of liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry for improving protein identification in shotgun proteome analysis., PMID:18479145
PI3K activation is associated with intracellular sodium/iodide symporter protein expression in breast cancer., PMID:17651485
Decidualized and pre-decidualized normal endometrial stromal cells produce more O-linked N-acetylglucosamine containing epitope H than non-decidualized normal endometrial stromal cells., PMID:16874662
Prohibitin is a novel target gene of vitamin D involved in its antiproliferative action in breast cancer cells., PMID:16849588
Proteomic analysis of ubiquitinated proteins from human MCF-7 breast cancer cells by immunoaffinity purification and mass spectrometry., PMID:16335966
Breast carcinoma cell lysate-pulsed dendritic cells cross-prime MUC1-specific CD8+ T cells identified by peptide-MHC-class-I tetramers., PMID:15919376
Reduced expression of the regulatory A subunit of serine/threonine protein phosphatase 2A in human breast cancer MCF-7 cells., PMID:14532964
Role of caspase 3-dependent Bcl-2 cleavage in potentiation of apoptosis by Bcl-2., PMID:11752215
Aromatic hydrocarbon receptor interaction with the retinoblastoma protein potentiates repression of E2F-dependent transcription and cell cycle arrest., PMID:10644764
Genistein-induced G2/M arrest is associated with the inhibition of cyclin B1 and the induction of p21 in human breast carcinoma cells., PMID:9664138
Correlation between DNA topoisomerase II activity and cytotoxicity in pMC540 and merodantoin sensitive and resistant human breast cancer cells., PMID:7762997
For research use only. Not for human or drug use.
