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Hela whole cell lysate

Catalog #:   CLH00045 Specific References (50) DATASHEET
Applications: WB
Overview

Catalog No.

CLH00045

Concentration

100 µg at 2 mg/ml

Applications

WB

Form

Liquid

Storage buffer

1 X Sample Buffer (1 X RIPA buffer + 1 X SDS loading buffer).

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4),150mM NaCl,1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Data Image
References

Proteome-Wide Profiling of Olaparib Interactors Using a Biotinylated Photoaffinity Probe., PMID:39898787

Development of a DNAzyme Walker for the Detection of APE1 in Living Cancer Cells., PMID:37725609

Design and synthesis of Aza-boeravinone derivatives as potential novel topoisomerase I inhibitors., PMID:35338969

A terminal alkyne and disulfide functionalized agarose resin specifically enriches azidohomoalanine labeled nascent proteins., PMID:33486215

A machine learning-based chemoproteomic approach to identify drug targets and binding sites in complex proteomes., PMID:32826910

Cell Proteins Interacting with the Human Immunodeficiency Virus in Immunoblotting can be Detected by R5- or X4- Tropic Human Immunodeficiency Virus Particles., PMID:32566522

A microfluidic paper-based laser-induced fluorescence sensor based on duplex-specific nuclease amplification for selective and sensitive detection of miRNAs in cancer cells., PMID:32456922

Identification of a novel anti‑heat shock cognate 71 kDa protein antibody in patients with Kawasaki disease., PMID:32319608

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling., PMID:31789314

Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation., PMID:31667293

Chlamydia trachomatis recruits protein kinase C during infection., PMID:31647538

Human RNF113A participates of pre-mRNA splicing in vitro., PMID:30506991

Whole Proteome Profiling of N-Myristoyltransferase Activity and Inhibition Using Sortase A., PMID:30341083

Improved Precursor Characterization for Data-Dependent Mass Spectrometry., PMID:29272103

MS Western, a Method of Multiplexed Absolute Protein Quantification is a Practical Alternative to Western Blotting., PMID:29192002

Tip-Based Fractionation of Batch-Enriched Phosphopeptides Facilitates Easy and Robust Phosphoproteome Analysis., PMID:29083192

Human recombinant Fab fragment from combinatorial libraries of a B-cell lymphoma patient recognizes core protein of chondroitin sulphate proteoglycan 4., PMID:29036679

A Multicenter Study To Evaluate the Performance of High-Throughput Sequencing for Virus Detection., PMID:28932815

Rational design of drug-like compounds targeting Mycobacterium marinum MelF protein., PMID:28873466

Tryptic Peptides Bearing C-Terminal Dimethyllysine Need to Be Considered during the Analysis of Lysine Dimethylation in Proteomic Study., PMID:28730820

Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification., PMID:28524877

High-Throughput Analysis of Intact Human Proteins Using UVPD and HCD on an Orbitrap Mass Spectrometer., PMID:28412815

Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer., PMID:28266123

HTP Nutraceutical Screening for Histone Deacetylase Inhibitors and Effects of HDACis on Tumor-suppressing miRNAs by Trichostatin A and Grapeseed (Vitis vinifera) in HeLa cells., PMID:28031235

TRAIL signaling is proinflammatory and proviral in a murine model of rhinovirus 1B infection., PMID:27836899

Stress granules and RNA processing bodies are novel autoantibody targets in systemic sclerosis., PMID:26801089

Interrogating Endogenous Protein Phosphatase Activity with Rationally Designed Chemosensors., PMID:26580981

Cross-linking immunoprecipitation-MS (xIP-MS): Topological Analysis of Chromatin-associated Protein Complexes Using Single Affinity Purification., PMID:26560067

Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines., PMID:26363641

Direct Monitoring of Nucleotide Turnover in Human Cell Extracts and Cells by Fluorogenic ATP Analogs., PMID:26274552

MStern Blotting-High Throughput Polyvinylidene Fluoride (PVDF) Membrane-Based Proteomic Sample Preparation for 96-Well Plates., PMID:26223766

Fluorescent image analysis of HIV-1 and HIV-2 uncoating kinetics in the presence of old world monkey TRIM5α., PMID:25803716

[Functional analysis of cancer-derived immunoglobulin G whole molecule-interacting proteins identified by LC-MS/MS]., PMID:25613617

Unexpected lack of specificity of a rabbit polyclonal TAP-L (ABCB9) antibody., PMID:27335633

Phosphorylation status of human RNA-binding protein 8A in cells and its inhibitory regulation by Magoh., PMID:25349214

Practical and Efficient Searching in Proteomics: A Cross Engine Comparison., PMID:25346847

Evaluation of data-dependent and -independent mass spectrometric workflows for sensitive quantification of proteins and phosphorylation sites., PMID:25330945

Osteoblastic protein tyrosine phosphatases inhibition and connexin 43 phosphorylation by alendronate., PMID:24698731

Identification and functional analysis of the cytolethal distending toxin gene from Avibacterium paragallinarum., PMID:24188584

BAD-lectins: boronic acid-decorated lectins with enhanced binding affinity for the selective enrichment of glycoproteins., PMID:23895469

Metformin inhibits heme oxygenase-1 expression in cancer cells through inactivation of Raf-ERK-Nrf2 signaling and AMPK-independent pathways., PMID:23707609

Ethanolic extract of the Goldenseal, Hydrastis canadensis, has demonstrable chemopreventive effects on HeLa cells in vitro: Drug-DNA interaction with calf thymus DNA as target., PMID:23628949

Functional proteomics strategy for validation of protein kinase inhibitors reveals new targets for a TBB-derived inhibitor of protein kinase CK2., PMID:23017496

Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones., PMID:22644042

Optimized fast and sensitive acquisition methods for shotgun proteomics on a quadrupole orbitrap mass spectrometer., PMID:22537090

Analysis of protein mixtures from whole-cell extracts by single-run nanoLC-MS/MS using ultralong gradients., PMID:22498708

Nano-LC FTICR tandem mass spectrometry for top-down proteomics: routine baseline unit mass resolution of whole cell lysate proteins up to 72 kDa., PMID:22356091

[Proteomic peptide library for determination of substrate motif of casein kinase 2]., PMID:22128731

A bead-based approach for large-scale identification of in vitro kinase substrates., PMID:22113938

Lamin A and lamin C form homodimers and coexist in higher complex forms both in the nucleoplasmic fraction and in the lamina of cultured human cells., PMID:22033280

Datasheet

Document Download

Hela whole cell lysate.pdf

 

$ 167
Product specifications
100 μg 167

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Hela whole cell lysate [CLH00045]
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